Fast identification of lipase inhibitors in oolong tea by using lipase functionalised Fe3O4 magnetic nanoparticles coupled with UPLC-MS/MS

	Fast identification of lipase inhibitors in oolong tea by using lipase functionalised Fe3O4 magnetic nanoparticles coupled with UPLC-MS/MS
	Zhu, Yuan-Ting; Ren, Xiao-Yun; Yuan, Li; Liu, Yi-Ming; Liang, Jian; Liao, Xun
刊名	FOOD CHEMISTRY
	2015
卷号	173 页码:521-526
关键词	POLYPHENOLS SELECTION LEAVES MS
通讯作者	Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China.
产权排序	1
合作状况	国际
英文摘要	Oolong tea is an important member in tea family, which claims for various health benefits such as preventing obesity and improving lipid metabolism. In this work, using pancreatic lipase (PL) functionalised magnetic nanoparticles (PL-MNPs) as solid phase extraction absorbent in combination with ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS), we developed a method for rapid screening and identification of lipase inhibitors from oolong tea. Three PL ligands were selectively extracted and identified as (-)-epigallocatechin-3-O-gallate (EGCG), (-)-gallocatechin-3-O-gallate (GCG) and (-)-epicatechin-3-O-gallate (ECG). Their lipase inhibitory activities were significantly higher than those non-ligands. Structure-activity analysis revealed that the presence of a galloyl moiety in the structure was required for binding to PL-MNPs, and therefore, exhibiting a strong inhibition on the enzyme. Taking advantages of the specificity in enzyme binding and the convenience of magnetic separation, this method has great potential for fast screening of lipase inhibitors from natural resources. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Chemistry; Food Science & Technology; Nutrition & Dietetics
类目[WOS]	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/27591]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Zhu, Yuan-Ting,Ren, Xiao-Yun,Yuan, Li,et al. Fast identification of lipase inhibitors in oolong tea by using lipase functionalised Fe3O4 magnetic nanoparticles coupled with UPLC-MS/MS[J]. FOOD CHEMISTRY,2015,173:521-526.
APA	Zhu, Yuan-Ting,Ren, Xiao-Yun,Yuan, Li,Liu, Yi-Ming,Liang, Jian,&Liao, Xun.(2015).Fast identification of lipase inhibitors in oolong tea by using lipase functionalised Fe3O4 magnetic nanoparticles coupled with UPLC-MS/MS.FOOD CHEMISTRY,173,521-526.
MLA	Zhu, Yuan-Ting,et al."Fast identification of lipase inhibitors in oolong tea by using lipase functionalised Fe3O4 magnetic nanoparticles coupled with UPLC-MS/MS".FOOD CHEMISTRY 173(2015):521-526.