A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway

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	A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway
	Zhang, Ran 1,4; Liu, Rui 1; Xin, Lusheng1,3 ; Chen, Hao1,3 ; Li, Chenghua 4; Wang, Lingling 2; Song, Linsheng 2
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2016-12-01
卷号	65 页码:98-106
关键词	Crassostrea gigas Interferon Interferon receptor Poly (I: C) Interferon stimulated genes
英文摘要	Interferon is a highly pleiotropic cytokine, once binding to its receptors, can activate JAK kinases and STAT transcription factors to initiate the transcription of genes downstream from interferon-stimulated response elements. In the present study, a cytokine receptor-like 3 molecule was selected and cloned from oyster Crassostrea gigas, which contained a fibronectin type III domain (designed CgIFNR-3). The expression pattern of CgIFNR-3 mRNA was detected in all the tested tissues including mantle, gills, hepatopancreas, muscle, and hemocytes, with the highest expression level in hemocytes. After poly (I: C) stimulation, the expression level of CgIFNR-3 in hemocytes was observed to significantly increase at 3 h (13.06-fold, p < 0.01). CgIFNR-3 was indicated to interact with CgIFNLP by in vitro GST pull-down assay, and to activate the expression of transcription factors including ISRE, STAT3 and GAS, in human Janus kinase signal transducer and activator of transcription (JAK/STAT) pathway after co-transfection in HEK-293T cells in the reporter luciferase activity assay. These results suggested that CgIFNR-3 could bind to CgIFNLP as an interferon receptor and participate in the activation of JAK/STAT pathway in human, which will benefit for intensive studies of interferon signaling pathway in mollusc. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000384388400011
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/135895]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ran,Liu, Rui,Xin, Lusheng,et al. A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:98-106.
APA	Zhang, Ran.,Liu, Rui.,Xin, Lusheng.,Chen, Hao.,Li, Chenghua.,...&Song, Linsheng.(2016).A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,98-106.
MLA	Zhang, Ran,et al."A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):98-106.