滨海湿地植物芦苇可培养内生真菌的多样性

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	滨海湿地植物芦苇可培养内生真菌的多样性
	李海林 ; 马斌 ; 张晓黎; 张倩倩; 郭晓红 ; 范德朋 ; 龚骏
刊名	应用生态学报
	2016-04-18
卷号	27 期号:7 页码:2066-2074
关键词	滨海湿地芦苇内生真菌盐度胁迫
ISSN号	1001-9332
其他题名	Diversity of culturable endophytic fungi of common reed ( Phragmites australis) in coastal wetland
通讯作者	龚骏
产权排序	中国科学院烟台海岸带研究所微型生物生态与物质循环实验室;中国科学院大学;佛山市碧沃丰生物科技股份有限公司;
中文摘要	内生真菌在植物生长以及抵御环境胁迫过程中起着非常重要的生态作用.本研究从黄河三角洲滨海湿地1350个芦苇组织切片中分离得到318株内生真菌,通过对rDNA ITS的分型、测序及系统进化分析,研究了该地区芦苇植株根、茎、叶中可培养内生真菌的种类组成及在不同盐度、不同组织中的分布情况.根据序列相似性(以98%为阈值),共获得12个真菌分类操作单元(OTUs).在门级分类水平上,子囊菌门为绝对优势菌群,在各组织和站位中均有分布.芦苇根组织中分离得到的内生真菌OTU数相对较多,叶组织和茎组织中分离到的OTU数相同,且叶中的OTU在根中均存在.潮上区(低盐区)内生真菌OTU数最多,高潮区次之.根中可培养...
英文摘要	Endophytic fungi play an important ecological role in promoting growth and alleviating environmental stress of host plants. In this study, we investigated the diversity and community composition of endophytic fungi isolated from the common reed in wetlands of the Yellow River Delta. A total of 318 endophytic fungal isolates were obtained from 1350 tissue (leaf, stem, and root) fragments and from three tidal zones with different soil salinity levels. rDNA ITS regions of these isolates were PCR amplified, sequenced, and phylogenetically analyzed. All the sequences were divided into 12 operational taxonomic units (OTUs) at the 98% similarity threshold. Ascomycota was found to be the most abundant flora at the level of the phylum and distributed in all tissues and sites. There were generally richer OTUs in roots than in leaves and stems, and leaves and stems shared the same OTU numbers. All the OTUs retrieved from leaves appeared in roots. The supra tide (low salinity zone) had the most OTUs among the three sampling sites, followed by high tide. Furthermore, the roots had the highest richness and diversity, whereas the leaves had the lowest; supra tide held the highest richness, and the middle tide had the highest diversity. Although unique OTUs could be found in different tissues and tidal zones of different salinity, the communities of culturable endophytic fungi were not substantially different among tissues or tidal zones (ANOSIM, P>0.05). Alternaria sp. was the shared dominant taxa among all samples.
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:5749532
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/17274]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.中国科学院烟台海岸带研究所微型生物生态与物质循环实验室 2.中国科学院大学 3.佛山市碧沃丰生物科技股份有限公司
推荐引用方式 GB/T 7714	李海林,马斌,张晓黎,等. 滨海湿地植物芦苇可培养内生真菌的多样性[J]. 应用生态学报,2016,27(7):2066-2074.
APA	李海林.,马斌.,张晓黎.,张倩倩.,郭晓红.,...&龚骏.(2016).滨海湿地植物芦苇可培养内生真菌的多样性.应用生态学报,27(7),2066-2074.
MLA	李海林,et al."滨海湿地植物芦苇可培养内生真菌的多样性".应用生态学报 27.7(2016):2066-2074.