Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda

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	Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda
	Ge, Qianqian 1; Liang, Junping 1; Li, Jitao 1; Li, Jian 1; Duan, Yafei 1; Zhao, Fazhen 1; Ren, Hai 1
刊名	FISHERIES SCIENCE
	2015
卷号	81 期号:4 页码:699-711
关键词	Exopalaemon carinicauda Relish Cloning Vibrio anguillarum WSSV Ammonia-N stress Expression
通讯作者	ge2682@126.com ; lijian@ysfri.ac.cn
英文摘要	Rel/NF-kappa B transcription factors play critical roles in induction and regulation of innate immune response in organisms. In this study, the full length of a Relish homolog cDNA from Exopalaemon carinicauda named EcRelish was 2141 bp encoding a 660 amino-acid polypeptide. EcRelish cDNA contained a conserved Rel homology domain and two nucleus localization signals. Sequence analysis indicated that the deduced amino acid sequence of the EcRelish showed high similarities to that of other crustaceans. Real time RT-PCR analysis showed that EcRelish mRNA expressed with different levels in tested tissues, and the highest expression was observed in the hemocytes. With longer infection time, the cumulative mortality rates increased gradually followed by the proliferation of Vibrio anguillarum and WSSV. The expression profiles of EcRelish gene were analyzed after V. a nguillarum, white spot syndrome virus (WSSV) challenge, and ammonia-N stress. The results showed that the expression levels of EcRelish mRNA in the hemocytes were up-regulated at 1-24 h after V. a nguillarum challenge. Meanwhile, the expression levels of EcRelish mRNA were up-regulated at 3 h after WSSV challenge. The expression of EcRelish in hemocytes was down-regulated significantly under ammonia-N stress during the experimental time. The results indicated that EcRelish might be involved in immune defense against pathogens and ammonia-N stress in E. c arinicauda.
学科主题	Fisheries
原文出处	0919-9268
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14809]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.[Ge, Qianqian] Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China 2.[Ge, Qianqian 3.Li, Jitao 4.Li, Jian 5.Zhao, Fazhen 6.Ren, Hai] Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 7.[Liang, Junping] Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Peoples R China 8.[Duan, Yafei] Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Ge, Qianqian,Liang, Junping,Li, Jitao,et al. Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda[J]. FISHERIES SCIENCE,2015,81(4):699-711.
APA	Ge, Qianqian.,Liang, Junping.,Li, Jitao.,Li, Jian.,Duan, Yafei.,...&Ren, Hai.(2015).Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda.FISHERIES SCIENCE,81(4),699-711.
MLA	Ge, Qianqian,et al."Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda".FISHERIES SCIENCE 81.4(2015):699-711.