Characterization and expression analysis of BCA2 gene in large yellow croaker, Larimichthys crocea

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	Characterization and expression analysis of BCA2 gene in large yellow croaker, Larimichthys crocea
	Zhang, Dong Ling 1; Yu, Da Hui 1; Chen, Jian 1; Wang, Zhi Yong 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015
卷号	46 期号:2 页码:543-549
关键词	BCA2 Larimichthys crocea Immune response In-situ hybridization E3 ubiquitin ligase
通讯作者	zywang@jmu.edu.cn
英文摘要	BCA2, as an E3 ubiquitin ligase, is an important anti-virus immune factor in mammals. Up to date, there are not any related reports on BCA2 protein in fishes yet. In the present investigation, BCA2 in large yellow croaker Larimichthys crocea (named as LcBCA2) was identified and characterized. The full-length cDNA of LcBCA2 was 1571 bp, including an ORF of 888 bp encoding a polypeptide of 295 amino acids. The putative LcBCA2 protein contained a RING-H2 motif at C terminal. The LcBCA2 transcripts were broadly distributed in all detected tissues, with high expression in muscle, moderate in blood, skin, heart, liver and spleen, weak in other tissue as indicated by qPCR analysis. Significant increases were observed in skin, gill and spleen after infection of Cryptocaryon irritans, and in spleen and head-kidney after inactivated Vibrio. parahaemolyticus, LPS and Poly I:C stimulations. Tissue localization by in-situ hybridization showed that LcBCA2 mainly expressed in the spleen of the fish in the test group. Our findings showed that LcBCA2 inclined to sharply increase in immune organs, especially in head-kidney after bacterial and viral stimulations, while in locations (skin and gill) of parasites infections, suggesting that BCA2 may play an important role in fish defense against bacteria, virus and parasites infections, but the immune mechanisms is are different. (C) 2015 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
原文出处	1050-4648
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14762]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.[Zhang, Dong Ling 2.Chen, Jian 3.Wang, Zhi Yong] Jimei Univ, Coll Fisheries, Minist Agr, Key Lab Hlth Mariculture East China Sea, Xiamen 361021, Fujian Province, Peoples R China 4.[Yu, Da Hui] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, South China Sea Resource Exploitat & Protect Coll, Guangzhou 510300, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dong Ling,Yu, Da Hui,Chen, Jian,et al. Characterization and expression analysis of BCA2 gene in large yellow croaker, Larimichthys crocea[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):543-549.
APA	Zhang, Dong Ling,Yu, Da Hui,Chen, Jian,&Wang, Zhi Yong.(2015).Characterization and expression analysis of BCA2 gene in large yellow croaker, Larimichthys crocea.FISH & SHELLFISH IMMUNOLOGY,46(2),543-549.
MLA	Zhang, Dong Ling,et al."Characterization and expression analysis of BCA2 gene in large yellow croaker, Larimichthys crocea".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):543-549.