Thioredoxin of golden pompano involved in the immune response to Photobacterium damselae

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	Thioredoxin of golden pompano involved in the immune response to Photobacterium damselae
	Wang, Long; Guo, Huayang; Zhang, Nan; Ma, Zhenhua; Jiang, Shigui; Zhang, Dianchang
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015
卷号	45 期号:2 页码:808-816
关键词	Thioredoxin Trachinotus ovatus mRNA expression Immune response Antioxidant function
通讯作者	zhangdch@163.com
中文摘要	Thioredoxin (TRX) is one of the key systems responsible for keeping the intracellular environment in a highly reduced state. In this study, a full-length TRX cDNA sequence (ToTRX) from golden pompano Trachinotus ovatus was identified after pyrosequencing of golden pompano cDNA library. ToTRX cDNA is comprised of 786 bp, and contained a 324 bp open reading frame (ORF) encoding a 107 amino acid polypeptide, a 5' untranslated region (UTR) of 116 bp, and a long 3'- UTR of 346 bp. Multiple sequence alignment revealed that ToTRX contained the highly conserved redox active disulphide/dithiol site (CGPC) of the thioredoxin active family, and phylogenetic tree showed that ToTRX had a closer evolution relationship with TRX from Oplegnathus fasciatus and Anoplopoma fimbria. ToTRX mRNA is ubiquitously expressed in all detected tissues with the higher expression levels in the stomach, gill and fin tissues. The expression of ToTRX mRNA was significantly up-regulated in liver, kidney, intestine and spleen of golden pompano injected with Photobacterium damselae. The recombinant ToTRX protein (rToTRX) was expressed in Escherichia coli BL21 (DE3), and then purified and refolded. The insulin disulfides assay was performed to investigate the enzymatic oxidoreductase activity of rToTRX, and the results demonstrated that rToTRX exhibited a high reducing activity in presence of DTT, while no activity was observed in the regroup without DTT and blank control group. Over all, the study provided the useful information to help further understand the functional mechanism of TRX in marine fish immunity. (C) 2015 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14942]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Wang, Long,Guo, Huayang,Zhang, Nan,et al. Thioredoxin of golden pompano involved in the immune response to Photobacterium damselae[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):808-816.
APA	Wang, Long,Guo, Huayang,Zhang, Nan,Ma, Zhenhua,Jiang, Shigui,&Zhang, Dianchang.(2015).Thioredoxin of golden pompano involved in the immune response to Photobacterium damselae.FISH & SHELLFISH IMMUNOLOGY,45(2),808-816.
MLA	Wang, Long,et al."Thioredoxin of golden pompano involved in the immune response to Photobacterium damselae".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):808-816.