A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079
	Chan, Zhuhua; Zhong, Tianhua; Yi, Zhiwei; Xiao, Jing; Zeng, Runying
刊名	BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
	2015
卷号	20 期号:5 页码:908-914
关键词	Streptomyces sp MC079 enduracidin pH-shift feeding strategy fed-batch fermentation
通讯作者	zeng@tio.org.cn
中文摘要	The aim of this work is to enhance enduracidin production by Streptomyces sp. MC079. Based on the time course analysis of the specific cell growth rate and specific enduracidin formation rate, a two-stage pH control strategy was proposed to improve enduracidin production by shifting the culture pH from 5.5 to 5.8 after 112 h of cultivation. By applying this pH control strategy, enduracidin concentration and productivity was 51.2 and 65.0% higher than results with uncontrolled pH batch fermentation. For further enhancement of enduracidin production, the effects of constant-rate feeding and pH-shift feeding strategy were investigated. The results indicated that the pH-shift feeding strategy increased the maximum concentration and productivity of enduracidin to 61.37 mg/L and 0.697 mg/L/h in the constant-rate feeding fermentation process. This is 73.3 and 88.9% higher than results with uncontrolled pH batch fermentation, respectively. The obtained optimal pH shift feeding strategy may be useful for the industrial-scale microbial production of enduracidin.
学科主题	Biotechnology & Applied Microbiology
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14916]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Chan, Zhuhua,Zhong, Tianhua,Yi, Zhiwei,et al. A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2015,20(5):908-914.
APA	Chan, Zhuhua,Zhong, Tianhua,Yi, Zhiwei,Xiao, Jing,&Zeng, Runying.(2015).A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,20(5),908-914.
MLA	Chan, Zhuhua,et al."A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 20.5(2015):908-914.