De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics
	Lin, Qiang; Luo, Wei; Wan, Shiming; Gao, Zexia
刊名	PLOS ONE
	2016
卷号	11 期号:4 页码:e0154096
通讯作者	Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510275, Guangdong, Peoples R China.
中文摘要	Seahorse conservation has been performed utilizing various strategies for many decades, and the deeper understanding of genomic information is necessary to more efficiently protect the germplasm resources of seahorse species. However, little genetic information about seahorses currently exists in the public databases. In this study, high-throughput RNA sequencing for two seahorse species, Hippocampus erectus and H. mohnikei, was carried out, and de novo assembly generated 37,506 unigenes for H. erectus and 36,113 unigenes for H. mohnikei. Among them, 17,338 (46.23%) unigenes for H. erectus and 17,900 (49.57%) for H. mohnikei were successfully annotated based on the information available from the public databases. Through comparing the unigenes of two seahorse species, 7,802 candidate orthologous genes were identified and 5,268 genes among them could be annotated. In addition, gene ontology analysis of two species was similarly performed on biological processes, cellular components, and molecular functions. Twenty-four and twenty-one unigenes in H. erectus and H. mohnikei were annotated in the biosynthesis of unsaturated fatty acids pathways, and both seahorses lacked the Delta 12 and Delta 15 desaturases. Total of 8,992 and 9,116 SSR loci were obtained from H. erectus and H. mohnikei unigenes, respectively. Dozens of SSR were developed and then applied to assess the population genetic diversity, as well as cross-amplified in a related species, H. trimaculatus. The H-O and H-E values of the tested populations for H. erectus, H. mohnikei, and H. trimaculatus were medium. These resources would facilitate the conservation of the species through a better understanding of the genomics and comparative genome analysis within the Hippocampus genus.
学科主题	Science & Technology - Other Topics
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15373]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Lin, Qiang,Luo, Wei,Wan, Shiming,et al. De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics[J]. PLOS ONE,2016,11(4):e0154096.
APA	Lin, Qiang,Luo, Wei,Wan, Shiming,&Gao, Zexia.(2016).De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics.PLOS ONE,11(4),e0154096.
MLA	Lin, Qiang,et al."De Novo Transcriptome Analysis of Two Seahorse Species (Hippocampus erectus and H. mohnikei) and the Development of Molecular Markers for Population Genetics".PLOS ONE 11.4(2016):e0154096.