Ribosome Inactivating Proteins from Rosaceae | |
Shang, Chenjing; Rouge, Pierre; Van Damme, Els J. M. | |
刊名 | MOLECULES |
2016 | |
卷号 | 21期号:8页码:1105 |
关键词 | carbohydrate binding activity molecular modeling protein synthesis inhibition ribosome-inactivating proteins |
通讯作者 | Van Damme, EJM (reprint author), Univ Ghent, Dept Mol Biotechnol, Fac Biosci Engn, B-9000 Ghent, Belgium. |
中文摘要 | Ribosome-inactivating proteins (RIPs) are widespread among higher plants of different taxonomic orders. In this study, we report on the RIP sequences found in the genome/transcriptome of several important Rosaceae species, including many economically important edible fruits such as apple, pear, peach, apricot, and strawberry. All RIP domains from Rosaceae share high sequence similarity with conserved residues in the catalytic site and the carbohydrate binding sites. The genomes of Malus domestica and Pyrus communis contain both type 1 and type 2 RIP sequences, whereas for Prunus mume, Prunus persica, Pyrus bretschneideri, and Pyrus communis a complex set of type 1 RIP sequences was retrieved. Heterologous expression and purification of the type 1 as well as the type 2 RIP from apple allowed to characterize the biological activity of the proteins. Both RIPs from Malus domestica can inhibit protein synthesis. Furthermore, molecular modelling suggests that RIPs from Rosaceae possess three-dimensional structures that are highly similar to the model proteins and can bind to RIP substrates. Screening of the recombinant type 2 RIP from apple on a glycan array revealed that this type 2 RIP interacts with terminal sialic acid residues. Our data suggest that the RIPs from Rosaceae are biologically active proteins. |
学科主题 | Chemistry |
内容类型 | 期刊论文 |
源URL | [http://ir.scsio.ac.cn/handle/344004/15343] |
专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
推荐引用方式 GB/T 7714 | Shang, Chenjing,Rouge, Pierre,Van Damme, Els J. M.. Ribosome Inactivating Proteins from Rosaceae[J]. MOLECULES,2016,21(8):1105. |
APA | Shang, Chenjing,Rouge, Pierre,&Van Damme, Els J. M..(2016).Ribosome Inactivating Proteins from Rosaceae.MOLECULES,21(8),1105. |
MLA | Shang, Chenjing,et al."Ribosome Inactivating Proteins from Rosaceae".MOLECULES 21.8(2016):1105. |
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