Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response
	Chen, Ting; Ren, Chunhua; Li, Wuhu; Jiang, Xiao; Xia, Jianjun; Wong, Nai-Kei; Hu, Chaoqun
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015
卷号	45 期号:2 页码:231-238
关键词	Calmodulin Gene structure Stichopus monotuberculatus Nitric oxide Invertebrate innate immunity Vibrio
通讯作者	chan1010@scsio.ac.cn ; rosemary166@sina.com ; liwuhusunny@163.com ; jiangxiao4367@126.com ; jianjunxia@scsio.ac.cn ; wongnk@gmail.com ; hucq@scsio.ac.cn
中文摘要	Calmodulin (CaM) is an essential second messenger protein that transduces calcium signals by binding calcium ions (Ca2+) and modulating its interactions with various target proteins. In contrast to vertebrates, where CaM is well established as a cofactor for Ca2+-dependent physiological and cellular functions including host defense, there is a paucity of understanding on CaM in invertebrates (such as echinoderms) in response to immune challenge or microbial infections. In this study, we obtained and described the gene sequence of CaM from the tropical sea cucumber Stichopus monotuberculatus, a promising yet poorly characterized aquacultural species. mRNA expression of StmCaM could be detected in the intestine and coelomic fluid after Vibrio alginolyticus injection. Transcriptional and translational expression of StmCaM was inducible in nature, as evidenced by the expression patterns in primary coelomocytes following Vibrio challenge. This response could be mimicked by the Vibrio cells membrane components or lipopolysaccharides (LPS), and blocked by co-treatment of the LPS-neutralizing agent polymyxin B (PMB). Furthermore, inhibition of CaM activity by incubation with its inhibitor tri-fluoroperazine dihydrochloride (TIP) blunted the production of Vibrio-induced nitric oxide (NO) and augmented the survival of invading Vibrio in coelomocytes. Collectively, our study here supplied the first evidence for echinoderm CaM participation in innate immunity, and provided a functional link between CaM expression and antibacterial NO production in sea cucumber. (C) 2015 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
收录类别	SCI
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15053]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Chen, Ting,Ren, Chunhua,Li, Wuhu,et al. Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):231-238.
APA	Chen, Ting.,Ren, Chunhua.,Li, Wuhu.,Jiang, Xiao.,Xia, Jianjun.,...&Hu, Chaoqun.(2015).Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response.FISH & SHELLFISH IMMUNOLOGY,45(2),231-238.
MLA	Chen, Ting,et al."Calmodulin of the tropical sea cucumber: Gene structure, inducible expression and contribution to nitric oxide production and pathogen clearance during immune response".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):231-238.