Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli

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	Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli
	Chen, Xiaoyan; Xu, Jingliang; Yang, Liu; Yuan, Zhenhong; Xiao, Shiyuan; Zhang, Yu; Liang, Cuiyi; He, Minchao; Guo, Ying
刊名	journal of industrial microbiology & biotechnology
	2015-11-01
卷号	42 期号:11 页码:1473-1479
关键词	3-methyl-1-butanol Isobutanol alpha-keto acid decarboxylase Alcohol dehydrogenase
英文摘要	higher alcohols, longer chain alcohols, contain more than 3 carbon atoms, showed close energy advantages as gasoline, and were considered as the next generation substitution for chemical fuels. higher alcohol biosynthesis by native microorganisms mainly needs gene expression of heterologous keto acid decarboxylase and alcohol dehydrogenases. in the present study, branched-chain alpha-keto acid decarboxylase gene from lactococcus lactis subsp. lactis cicc 6246 (kivd) and alcohol dehydrogenases gene from zymomonas mobilis cicc 41465 (adhb) were transformed into escherichia coli for higher alcohol production. sds-page results showed these two proteins were expressed in the recombinant strains. the resulting strain was incubated in lb medium at 37 a degrees c in erlenmeyer flasks and much more 3-methyl-1-butanol (104 mg/l) than isobutanol (24 mg/l) was produced. however, in 5 g/l glucose-containing medium, the production of two alcohols was similar, 156 and 161 mg/l for c4 (isobutanol) and c5 (3-methyl-1-butanol) alcohol, respectively. effects of fermentation factors including temperature, glucose content, and alpha-keto acid on alcohol production were also investigated. the increase of glucose content and the adding of alpha-keto acids facilitated the production of c4 and c5 alcohols. the enzyme activities of pure kivd on alpha-ketoisovalerate and alpha-ketoisocaproate were 26.77 and 21.24 mu mol min(-1) mg(-1), respectively. due to its ability on decarboxylation of alpha-ketoisovalerate and alpha-ketoisocaproate, the recombinant e. coli strain showed potential application on isoamyl alcohol and isobutanol production.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	keto acid decarboxylase ; saccharomyces-cerevisiae ; isobutanol production ; lactococcus-lactis ; phenylpyruvate decarboxylase ; corynebacterium-glutamicum ; biosynthesis ; metabolism ; enzyme ; identification
收录类别	SCI
语种	英语
WOS记录号	WOS:000362964900005
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/10873]
专题	中国科学院广州能源研究所
作者单位	Chinese Acad Sci, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xiaoyan,Xu, Jingliang,Yang, Liu,et al. Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli[J]. journal of industrial microbiology & biotechnology,2015,42(11):1473-1479.
APA	Chen, Xiaoyan.,Xu, Jingliang.,Yang, Liu.,Yuan, Zhenhong.,Xiao, Shiyuan.,...&Guo, Ying.(2015).Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli.journal of industrial microbiology & biotechnology,42(11),1473-1479.
MLA	Chen, Xiaoyan,et al."Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli".journal of industrial microbiology & biotechnology 42.11(2015):1473-1479.