Synthesis of the Hemoglobin-Conjugated Polymer Micelles by Thiol Michael Addition Reactions

	Synthesis of the Hemoglobin-Conjugated Polymer Micelles by Thiol Michael Addition Reactions
	Qi, Yanxin 1; Li, Taihang 1; Wang, Yupeng 1,2; Wei, Xing 1,2; Li, Bin 3; Chen, Xuesi 4; Xie, Zhigang 1; Jing, Xiabin 1; Huang, Yubin 1
刊名	MACROMOLECULAR BIOSCIENCE
	2016-06-01
卷号	16 期号:6 页码:906-913
关键词	hemoglobin oxygen carriers protein delivery thiol Michael addition
英文摘要	Amphiphilic triblock copolymers mPEG-b-PMAC-b-PCL are synthesized using methoxyl poly(ethylene glycol), cyclic carbonic ester monomer including acryloyl group, and e-caprolactone. Copolymers are self-assembled into core-shell micelles in aqueous solution. Thiolated hemoglobin (Hb) is conjugated with micelles sufficiently through thiol Michael addition reaction to form hemoglobin nanoparticles (HbNs) with 200 nm in diameter. The conjugation of Hb onto the micelle surface is further confirmed by X-ray photoelectron spectroscopy. Feeding ratio of copolymer micelles to Hb at 1: 3 would lead to the highest hemoglobin loading efficiency 36.7 wt%. The UV results demonstrate that the gas transporting capacity of HbNs is well remained after Hb is conjugated with polymeric micelles. Furthermore, the obtained HbNs have no obvious detrimental effects on blood components in vitro. This system may thus have great potential as one of the candidates to be developed as oxygen carriers and provide a reference for the modification of protein drugs.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/35181]
专题	化学研究所_高分子物理与化学实验室
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.SUNY Albany, Dept Chem, Albany, NY 12222 USA 4.Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China
推荐引用方式 GB/T 7714	Qi, Yanxin,Li, Taihang,Wang, Yupeng,et al. Synthesis of the Hemoglobin-Conjugated Polymer Micelles by Thiol Michael Addition Reactions[J]. MACROMOLECULAR BIOSCIENCE,2016,16(6):906-913.
APA	Qi, Yanxin.,Li, Taihang.,Wang, Yupeng.,Wei, Xing.,Li, Bin.,...&Huang, Yubin.(2016).Synthesis of the Hemoglobin-Conjugated Polymer Micelles by Thiol Michael Addition Reactions.MACROMOLECULAR BIOSCIENCE,16(6),906-913.
MLA	Qi, Yanxin,et al."Synthesis of the Hemoglobin-Conjugated Polymer Micelles by Thiol Michael Addition Reactions".MACROMOLECULAR BIOSCIENCE 16.6(2016):906-913.