Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides

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	Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides
	Shen, Rui 1,2; Li, Hong-Qiang 1; Zhang, Jie 2; Xu, Jian 1
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2016-07-01
卷号	179 期号:5 页码:740-752
关键词	Xylan Enzymatic hydrolysis Xylo-oligosaccharides Sodium chloride Ethanol Pigment
ISSN号	0273-2289
英文摘要	As the second abundant natural carbohydrate, xylan is normally prepared through alkaline extraction and then used for xylo-oligosaccharides (XOS) production. However, the extracted xylan inevitably contains salt, ethanol, and pigment. In order to investigate the effects of these impurities on XOS production, the alkaline-extracted xylan with different kinds and concentrations of impurities was made and then hydrolyzed using alkaline xylanase (EC 3.2.1.8) to produce XOS. The results showed that a certain concentration of salt (NaCl) promoted the XOS production, while ethanol and pigment inhibited the enzymatic hydrolysis process significantly. The color value mainly ascribed to the phenolic compounds binding to xylan was a key restriction factor in the enzymatic hydrolysis later stage. Using optimal xylan sample (with 10 mg/mL NaCl, color value of 4.6 x 10(5), without ethanol) as substrate, the highest XOS yield of 58.58 % was obtained. As the substrate of XOS production, prepared xylan should contain colored materials and ethanol as less as possible, however, retains appropriate salt.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	LIGNOCELLULOSIC BIOMASS ; XYLOOLIGOSACCHARIDES ; PURIFICATION ; MANUFACTURE ; CELLULOSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000379755800005
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/21232]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Univ Chem Technol, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Shen, Rui,Li, Hong-Qiang,Zhang, Jie,et al. Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,179(5):740-752.
APA	Shen, Rui,Li, Hong-Qiang,Zhang, Jie,&Xu, Jian.(2016).Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,179(5),740-752.
MLA	Shen, Rui,et al."Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 179.5(2016):740-752.