Scale-up laccase production from Trametes versicolor stimulated by vanillic acid

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	Scale-up laccase production from Trametes versicolor stimulated by vanillic acid
	Wang, Ke-Feng 1,2; Hu, Jian-Hua 1,2; Guo, Chen 1,2; Liu, Chun-Zhao 1
刊名	BIOPROCESS AND BIOSYSTEMS ENGINEERING
	2016-07-01
卷号	39 期号:7 页码:1041-1049
关键词	Trametes versicolor Vanillic acid Scale-up culture Laccase isoenzymes lcc
ISSN号	1615-7591
英文摘要	An efficient strategy for laccase production in Trametes versicolor cultures was developed using vanillic acid as the inducer. The optimized vanillic acid treatment strategy consisted of exposing 2-day-old mycelia cultures to 80 mg/L vanillic acid. After 4 days, laccase activity of 588.84 U/L was achieved in flasks which represented a 1.79-fold increase compared to the control. In 200-L airlift bioreactor, the maximal laccase activity reached up to 785.12 U/L using the optimized vanillic acid treatment strategy. The zymograms of culture supernatants revealed three bands with laccase activity, among which Lac1 and Lac2 were abundant laccase isoforms constitutively expressed, and Lac3 was an inducible isozyme by vanillic acid. The results of real-time quantitative PCR showed that the transcription level of lcc in T. versicolor cultures grown with vanillic acid for 7 days was about 5.64-fold greater than that without vanillic acid in flasks. In 200-L airlift bioreactor cultures of T. versicolor with addition of vanillic acid, the transcript level of lcc at day 7 was 2.62-fold higher than that in flasks with vanillic acid due to the good mass transfer and oxygen supply in the bioreactor system. This study provides a basis for understanding the induction mechanism of vanillic acid for laccase production and has good potential for industrial applications.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	PLEUROTUS-SAJOR-CAJU ; GENE-EXPRESSION ; AROMATIC-COMPOUNDS ; DIFFERENTIAL REGULATION ; SUBMERGED CULTURES ; FUNGI ; DECOLORIZATION ; OPTIMIZATION ; INDUCTION ; DYES
收录类别	SCI
语种	英语
WOS记录号	WOS:000378566700003
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/21178]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ke-Feng,Hu, Jian-Hua,Guo, Chen,et al. Scale-up laccase production from Trametes versicolor stimulated by vanillic acid[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2016,39(7):1041-1049.
APA	Wang, Ke-Feng,Hu, Jian-Hua,Guo, Chen,&Liu, Chun-Zhao.(2016).Scale-up laccase production from Trametes versicolor stimulated by vanillic acid.BIOPROCESS AND BIOSYSTEMS ENGINEERING,39(7),1041-1049.
MLA	Wang, Ke-Feng,et al."Scale-up laccase production from Trametes versicolor stimulated by vanillic acid".BIOPROCESS AND BIOSYSTEMS ENGINEERING 39.7(2016):1041-1049.