Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses

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	Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses
	Liu, Qi 1,2; Jia, Jilei 1,2; Yang, Tingyuan 1; Fan, Qingze 1,2; Wang, Lianyan 1; Ma, Guanghui 1,3
刊名	SMALL
	2016-04-06
卷号	12 期号:13 页码:1744-1757
关键词	dopamine polymerization pathogen-mimicking polymeric nanoparticles surface modification vaccine delivery and adjuvant systems
ISSN号	1613-6810
英文摘要	Aiming to enhance the immunogenicity of subunit vaccines, a novel antigen delivery and adjuvant system based on dopamine polymerization on the surface of poly(d,l-lactic-glycolic-acid) nanoparticles (NPs) with multiple mechanisms of immunity enhancement is developed. The mussel-inspired biomimetic polydopamine (pD) not only serves as a coating to NPs but also functionalizes NP surfaces. The method is facile and mild including simple incubation of the preformed NPs in the weak alkaline dopamine solution, and incorporation of hepatitis B surface antigen and TLR9 agonist unmethylated cytosine-guanine (CpG) motif with the pD surface. The as-constructed NPs possess pathogen-mimicking manners owing to their size, shape, and surface molecular immune-activating properties given by CpG. The biocompatibility and biosafety of these pathogen-mimicking NPs are confirmed using bone marrow-derived dendritic cells. Pathogen-mimicking NPs hold great potential as vaccine delivery and adjuvant system due to their ability to: 1) enhance cytokine secretion and immune cell recruitment at the injection site; 2) significantly activate and maturate dendritic cells; 3) induce stronger humoral and cellular immune responses in vivo. Furthermore, this simple and versatile dopamine polymerization method can be applicable to endow NPs with characteristics to mimic pathogen structure and function, and manipulate NPs for the generation of efficacious vaccine adjuvants.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]	DENDRITIC CELLS ; INNATE IMMUNITY ; IN-VIVO ; SURFACE MODIFICATION ; BACTERIAL-DNA ; HIV-1 P24 ; CPG DNA ; DELIVERY ; POLYDOPAMINE ; ANTIGEN
收录类别	SCI
语种	英语
WOS记录号	WOS:000373733200010
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/20982]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chem Sci & Engn Tianjin, Collaborat Innovat Ctr, Tianjin 300072, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qi,Jia, Jilei,Yang, Tingyuan,et al. Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses[J]. SMALL,2016,12(13):1744-1757.
APA	Liu, Qi,Jia, Jilei,Yang, Tingyuan,Fan, Qingze,Wang, Lianyan,&Ma, Guanghui.(2016).Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses.SMALL,12(13),1744-1757.
MLA	Liu, Qi,et al."Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses".SMALL 12.13(2016):1744-1757.