Protective effect of the essential oil of Zanthoxylum myriacanthum var. pubescens against dextran sulfate sodium-induced intestinal inflammation in mice

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 民族植物学研究组

	Protective effect of the essential oil of Zanthoxylum myriacanthum var. pubescens against dextran sulfate sodium-induced intestinal inflammation in mice
	Xu, You-Kai
刊名	PHYTOMEDICINE
	2016
卷号	23 期号:9 页码:883-890
关键词	Zanthoxylum myriacanthum var. pubescens Essential oil IBD DSS Intestinal inflammation Anti-inflammatory
中文摘要	Background: Zanthoxylum myriacanthum var. pubescens is an ethnic medicine for digestive disease known as Maqian. A previous report showed that the Maqian fruits essential oil (MQEO) exhibited an NO inhibitory effect on RAW 264.7 cells, but the effect on inflammatory disease in vivo remains unknown. Purpose: To investigate the anti-inflammatory effect of Z. myriacanthum var. pubescens as potential candidate for the treatment of intestinal inflammation. Study design: Evaluation of anti-inflammatory effect of MQEO using dextran sulfate sodium (DSS)-induced intestinal inflammation in mice and exploration of the mechanisms with THP-1 cells. Methods: C57BL/6 mice were provided drinking water containing 3% DSS for 10 days followed by normal drinking water for 3 days. MQEO (35 and 70 mg/kg) were given 5 days before experiments and continued for another 13 days. At the end of experiments, mice were euthanized and colonic tissue was collected to be analyzed by H&E staining, RT-PCR and immunohistochemistry for evaluating the damage of colons, the mRNA levels of IL-1 beta, IL-6, IL-12p35 and TNF-alpha, and the expressions of myeloperoxidase (MPO) and matrix metalloproteinase-9 (MMP-9). The LPS-stimulated THP-1 cell line was used for exploring the role of inflammatory markers using ELISA, western blot and flow cytometry methods. Results: Oral administration of MQEO (35 and 70 mg/kg) markedly attenuated the symptoms of intestinal inflammation, including diarrhea, rectal bleeding, and loss of body weight. It also reduced the shortening of colon length and histopathological damage. The expressions of MPO and MMP-9 and the mRNA levels of pro-inflammatory cytokines (IL-1 beta, IL-6 and IL-12p35) in colonic tissue significantly decreased after MQEQ treatment. The activation of NF-kappa B p65 in colonic mucosa was also markedly suppressed. In addition, MQEO significantly suppressed LPS-stimulated production of TNF-alpha and IL-1 beta, effectively blocked phosphorylation of IKK and I kappa B, and dose-dependently reduced LPS-stimulated expression of TLR4 in THP-1 cells at concentrations ranging from 0.01 parts per thousand to 0.05 parts per thousand (v/v). Conclusion: MQEO exhibited protective effect against DSS-induced intestinal inflammation and the antiinflammatory activity may be associated with TLR4 mediated NF-kappa B signaling pathway, suggesting it might be used as an anti-inflammatory agent.
公开日期	2016-08-11
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/9993]
专题	西双版纳热带植物园_民族植物学研究组
推荐引用方式 GB/T 7714	Xu, You-Kai. Protective effect of the essential oil of Zanthoxylum myriacanthum var. pubescens against dextran sulfate sodium-induced intestinal inflammation in mice[J]. PHYTOMEDICINE,2016,23(9):883-890.
APA	Xu, You-Kai.(2016).Protective effect of the essential oil of Zanthoxylum myriacanthum var. pubescens against dextran sulfate sodium-induced intestinal inflammation in mice.PHYTOMEDICINE,23(9),883-890.
MLA	Xu, You-Kai."Protective effect of the essential oil of Zanthoxylum myriacanthum var. pubescens against dextran sulfate sodium-induced intestinal inflammation in mice".PHYTOMEDICINE 23.9(2016):883-890.