Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure
	Mao, XH; Simon, AJ; Pei, H; Shi, JY; Li, J; Huang, Q; Plaxco, KW; Fan, CH
刊名	CHEMICAL SCIENCE
	2016
卷号	7 期号:2 页码:1200—1204
关键词	COMPUTATIONAL DESIGN PEROXIDASE-ACTIVITY PROTEIN ORIGAMI ASSEMBLIES SWITCHES INSIGHTS RELEASE COMPLEX SENSORS
ISSN号	2041-6520
通讯作者	Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai, Peoples R China. ; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai, Peoples R China. ; Shi, JY (reprint author), Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England. ; Shi, JY (reprint author), UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England. ; Fan, CH (reprint author), ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201200, Peoples R China.
英文摘要	Recognition of the fundamental importance of allosteric regulation in biology dates back to not long after its discovery in the 1960s. Our ability to rationally engineer this potentially useful property into normally non-allosteric catalysts, however, remains limited. In response we report a DNA nanotechnology-enabled approach for introducing allostery into catalytic nucleic acids. Specifically, we have grafted one or two copies of a peroxidase-like DNAzyme, hemin-bound G-quadruplex (hemin-G), onto a DNA tetrahedral nanostructure in such a manner as to cause them to interact, modulating their catalytic activity. We achieve allosteric regulation of these catalysts by incorporating dynamically responsive oligonucleotides that respond to specific "effector" molecules (complementary oligonucleotides or small molecules), altering the spacing between the catalytic sites and thus regulating their activity. This designable approach thus enables subtle allosteric modulation in DNAzymes that is potentially of use for nanomedicine and nanomachines.
收录类别	SCI
语种	英语
WOS记录号	WOS:000368835300045
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/25641]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Mao, XH,Simon, AJ,Pei, H,et al. Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure[J]. CHEMICAL SCIENCE,2016,7(2):1200—1204.
APA	Mao, XH.,Simon, AJ.,Pei, H.,Shi, JY.,Li, J.,...&Fan, CH.(2016).Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure.CHEMICAL SCIENCE,7(2),1200—1204.
MLA	Mao, XH,et al."Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure".CHEMICAL SCIENCE 7.2(2016):1200—1204.