Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis

CORC > 天津工业生物技术研究所 > 微生物生理与蛋白质组学张大伟 > 期刊论文

	Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis
	Sone, Yafeng 1,2; Nikoloff, Jonas M.1; Zhang, Dawei 1,2,3
刊名	JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
	2015-07-01
卷号	25 期号:7 页码:963-977
关键词	Promoter ribosome-binding site signal peptide Sec pathway Tat pathway ATP-binding cassette transporter
英文摘要	The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression owing to its single membrane and high capacity of secretion, simplifying the downstream processing of secretory proteins. During the last few years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of life science, such as enzyme production, feed additives, and food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as a highly promising expression system illuminating strong chemical- and temperature-inducible and other types of promoters, strategies for ribosome-binding-site utilization, and the novel approach of signal peptide selection. Furthermore, we outline the main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we introduce the latest discoveries of Tat-related complex structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies for improving the yield of intended productions. We expect this to promote striking future developments in the optimization and application of this bacterium.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	TWIN-ARGININE TRANSLOCATION ; SIGNAL RECOGNITION PARTICLE ; GRAM-POSITIVE BACTERIA ; THIOL-DISULFIDE OXIDOREDUCTASES ; METHYL PARATHION HYDROLASE ; RIBOSOME BINDING-SITES ; MESSENGER-RNA DECAY ; ABC TRANSPORTER ; HETEROLOGOUS PROTEINS ; RECOMBINANT PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000358701800002
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1506]
专题	天津工业生物技术研究所_微生物生理与蛋白质组学张大伟_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Sone, Yafeng,Nikoloff, Jonas M.,Zhang, Dawei. Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2015,25(7):963-977.
APA	Sone, Yafeng,Nikoloff, Jonas M.,&Zhang, Dawei.(2015).Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,25(7),963-977.
MLA	Sone, Yafeng,et al."Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 25.7(2015):963-977.