Activating Phosphoenolpyruvate Carboxylase and Phosphoenolpyruvate Carboxykinase in Combination for Improvement of Succinate Production

	Activating Phosphoenolpyruvate Carboxylase and Phosphoenolpyruvate Carboxykinase in Combination for Improvement of Succinate Production
	Tan, Zaigao 1,2,3; Zhu, Xinna 1,2; Chen, Jing 1,2,4; Li, Qingyan 1,2; Zhang, Xueli 1,2
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2013-08-01
卷号	79 期号:16 页码:4838-4844
英文摘要	Phosphoenolpyruvate (PEP) carboxylation is an important step in the production of succinate by Escherichia coli. Two enzymes, PEP carboxylase (PPC) and PEP carboxykinase (PCK), are responsible for PEP carboxylation. PPC has high substrate affinity and catalytic velocity but wastes the high energy of PEP. PCK has low substrate affinity and catalytic velocity but can conserve the high energy of PEP for ATP formation. In this work, the expression of both the ppc and pck genes was modulated, with multiple regulatory parts of different strengths, in order to investigate the relationship between PPC or PCK activity and succinate production. There was a positive correlation between PCK activity and succinate production. In contrast, there was a positive correlation between PPC activity and succinate production only when PPC activity was within a certain range; excessive PPC activity decreased the rates of both cell growth and succinate formation. These two enzymes were also activated in combination in order to recruit the advantages of each for the improvement of succinate production. It was demonstrated that PPC and PCK had a synergistic effect in improving succinate production.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	ESCHERICHIA-COLI-C ; METABOLIC EVOLUTION ; ENHANCED PRODUCTION ; STRAINS ; ACID ; OVEREXPRESSION ; EXPRESSION ; ZYMOMONAS ; BACTERIUM ; PATHWAYS
收录类别	SCI
语种	英语
WOS记录号	WOS:000322161700008
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1286]
专题	天津工业生物技术研究所_微生物代谢工程张学礼_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Tianjin Univ Sci & Technol, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Tan, Zaigao,Zhu, Xinna,Chen, Jing,et al. Activating Phosphoenolpyruvate Carboxylase and Phosphoenolpyruvate Carboxykinase in Combination for Improvement of Succinate Production[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2013,79(16):4838-4844.
APA	Tan, Zaigao,Zhu, Xinna,Chen, Jing,Li, Qingyan,&Zhang, Xueli.(2013).Activating Phosphoenolpyruvate Carboxylase and Phosphoenolpyruvate Carboxykinase in Combination for Improvement of Succinate Production.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,79(16),4838-4844.
MLA	Tan, Zaigao,et al."Activating Phosphoenolpyruvate Carboxylase and Phosphoenolpyruvate Carboxykinase in Combination for Improvement of Succinate Production".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 79.16(2013):4838-4844.