Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement

CORC > 天津工业生物技术研究所 > 天然化合物合成生物学实验室刘涛 > 期刊论文

	Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement
	Yin, Hua; Zhuang, Yi-bin; Li, E-e; Bi, Hui-ping; Zhou, Wei; Liu, Tao
刊名	BIOTECHNOLOGY LETTERS
	2015-06-01
卷号	37 期号:6 页码:1249-1255
关键词	Costunolide E. coli Farnesyl diphosphate Heterologous biosynthesis Mevalonate pathway Radix aucklandiae Sesquiterpene lactones (SLs)
英文摘要	Costunolide, the main bioactive compound of the medicinal plant, Radix Aucklandiae, is a sesquiterpene lactone (SL) and has a broad range of biological activities. It is also a precursor of many biologically-active SLs and is a branching point in the biosynthesis of SLs. Here we have reconstituted the costunolide biosynthetic pathway in Escherichia coli by co-expression of three genes (GAS, GAO, LsCOS) involved in costunolide biosynthesis and eight genes involved in converting acetyl-CoA into farnesyl diphosphate from mevalonate pathway. Costunolide production was then detected. By screening and optimization of cultured medium and inducing temperature, costunolide yield was up to 100 mg l(-1) in E. coli.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	SESQUITERPENE LACTONES ; IDENTIFICATION ; PARTHENOLIDE ; EXPRESSION ; CHICORY ; CELLS ; PLANT ; L.
收录类别	SCI
语种	英语
WOS记录号	WOS:000354498900015
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1462]
专题	天津工业生物技术研究所_天然化合物合成生物学实验室刘涛_期刊论文
作者单位	Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Yin, Hua,Zhuang, Yi-bin,Li, E-e,et al. Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement[J]. BIOTECHNOLOGY LETTERS,2015,37(6):1249-1255.
APA	Yin, Hua,Zhuang, Yi-bin,Li, E-e,Bi, Hui-ping,Zhou, Wei,&Liu, Tao.(2015).Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement.BIOTECHNOLOGY LETTERS,37(6),1249-1255.
MLA	Yin, Hua,et al."Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement".BIOTECHNOLOGY LETTERS 37.6(2015):1249-1255.