Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase

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	Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase
	Liu, Weidong; Guo, Rey-Ting; Chen, Xi; Li, Zhe; Gao, Xiuzhen; Huang, Chun-Hsiang; Wu, Qiaqing; Feng, Jinhui; Zhu, Dunming
刊名	CHEMBIOCHEM
	2015-04-13
卷号	16 期号:6 页码:924-929
关键词	amination dehydrogenases enzyme catalysis protein structures structural biology
英文摘要	A meso-diaminopimelate dehydrogenase (DAPDH) from Clostridium tetani E88 (CtDAPDH) was found to have low activity toward the D-amino acids other than its native substrate. Site-directed mutagenesis similar to that carried out on the residues mutated by Vedha-Peters et al. resulted in a mutant enzyme with highly improved catalytic ability for the synthesis of D-amino acids. The crystal structures of the CtDAPDH mutant in apo form and in complex with meso-diaminopimelate (meso-DAP), D-leucine (D-leu), and 4-methyl-2-oxopentanoic acid (MOPA) were solved. meso-DAP was found in an area outside the catalytic cavity; this suggested a possible two-step substrate-binding mechanism for meso-DAP. D-leu and MOPA each bound both to Leu154 and to Gly155 in the open form of CtDAPDH, and structural analysis revealed the molecular basis for the expanded substrate specificity of the mutant meso-diaminopimelate dehydrogenases.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Medicinal
研究领域[WOS]	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]	AMINO-ACID DEHYDROGENASE ; CORYNEBACTERIUM-GLUTAMICUM ; SYMBIOBACTERIUM-THERMOPHILUM ; 3-DIMENSIONAL STRUCTURE ; CRYSTALLOGRAPHY ; MICROBIOLOGY ; MUTAGENESIS ; REFINEMENT ; NUTRITION ; CREATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000352489300006
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1453]
专题	天津工业生物技术研究所_生物催化与绿色化工朱敦明_期刊论文
作者单位	Chinese Acad Sci, Ind Enzymes Natl Engn Lab, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Liu, Weidong,Guo, Rey-Ting,Chen, Xi,et al. Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase[J]. CHEMBIOCHEM,2015,16(6):924-929.
APA	Liu, Weidong.,Guo, Rey-Ting.,Chen, Xi.,Li, Zhe.,Gao, Xiuzhen.,...&Zhu, Dunming.(2015).Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase.CHEMBIOCHEM,16(6),924-929.
MLA	Liu, Weidong,et al."Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase".CHEMBIOCHEM 16.6(2015):924-929.