Global Microarray Analysis of Carbohydrate Use in Alkaliphilic Hemicellulolytic Bacterium Bacillus sp N16-5

	Global Microarray Analysis of Carbohydrate Use in Alkaliphilic Hemicellulolytic Bacterium Bacillus sp N16-5
	Song, Yajian 1,2,3; Xue, Yanfen 1,2; Ma, Yanhe 1,2
刊名	PLOS ONE
	2013-01-10
卷号	8 期号:1
英文摘要	The alkaliphilic hemicellulolytic bacterium Bacillus sp. N16-5 has a broad substrate spectrum and exhibits the capacity to utilize complex carbohydrates such as galactomannan, xylan, and pectin. In the monosaccharide mixture, sequential utilization by Bacillus sp. N16-5 was observed. Glucose appeared to be its preferential monosaccharide, followed by fructose, mannose, arabinose, xylose, and galactose. Global transcription profiles of the strain were determined separately for growth on six monosaccharides (glucose, fructose, mannose, galactose, arabinose, and xylose) and four polysaccharides (galactomannan, xylan, pectin, and sodium carboxymethylcellulose) using one-color microarrays. Numerous genes potentially related to polysaccharide degradation, sugar transport, and monosaccharide metabolism were found to respond to a specific substrate. Putative gene clusters for different carbohydrates were identified according to transcriptional patterns and genome annotation. Identification and analysis of these gene clusters contributed to pathway reconstruction for carbohydrate utilization in Bacillus sp. N16-5. Several genes encoding putative sugar transporters were highly expressed during growth on specific sugars, suggesting their functional roles. Two phosphoenolpyruvate-dependent phosphotransferase systems were identified as candidate transporters for mannose and fructose, and a major facilitator superfamily transporter was identified as a candidate transporter for arabinose and xylose. Five carbohydrate uptake transporter 1 family ATP-binding cassette transporters were predicted to participate in the uptake of hemicellulose and pectin degradation products. Collectively, microarray data improved the pathway reconstruction involved in carbohydrate utilization of Bacillus sp. N16-5 and revealed that the organism precisely regulates gene transcription in response to fluctuations in energy resources.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	DIFFERENT CARBON-SOURCES ; CLOSTRIDIUM-THERMOCELLUM ; THERMOTOGA-MARITIMA ; ABC TRANSPORTERS ; FUEL ETHANOL ; CELLULOSOME ; EXPRESSION ; ENZYMES ; BIOMASS ; DEGRADATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000313552400077
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1299]
专题	天津工业生物技术研究所_酶工程实验室马延和 _期刊论文
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Natl Engn Lab Ind Enzymes, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Song, Yajian,Xue, Yanfen,Ma, Yanhe. Global Microarray Analysis of Carbohydrate Use in Alkaliphilic Hemicellulolytic Bacterium Bacillus sp N16-5[J]. PLOS ONE,2013,8(1).
APA	Song, Yajian,Xue, Yanfen,&Ma, Yanhe.(2013).Global Microarray Analysis of Carbohydrate Use in Alkaliphilic Hemicellulolytic Bacterium Bacillus sp N16-5.PLOS ONE,8(1).
MLA	Song, Yajian,et al."Global Microarray Analysis of Carbohydrate Use in Alkaliphilic Hemicellulolytic Bacterium Bacillus sp N16-5".PLOS ONE 8.1(2013).