Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-beta-D-glucanase from Aspergillus aculeatus F-50

CORC > 天津工业生物技术研究所 > 结构生物学与蛋白酶学实验室郭瑞庭 > 期刊论文

	Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-beta-D-glucanase from Aspergillus aculeatus F-50
	Chen, Yun 1; Huang, Jian-Wen 2,3; Chen, Chun-Chi 4; Lai, Hui-Lin 2,3; Jin, Jian 1; Guo, Rey-Ting 4
刊名	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
	2015-04-01
卷号	71 期号:71 页码:397-400
关键词	cellulase endoglucanase Aspergillus industrial enzyme
英文摘要	Cellulose is the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in a wide variety of industries. Endo-1,4-beta-D-glucanase (EC 3.2.1.4; endoglucanase), which can catalyze the random hydrolysis of beta-1,4-glycosidic bonds to cleave cellulose into smaller fragments, is a key cellulolytic enzyme. An endoglucanase isolated from Aspergillus aculeatus F-50 (FI-CMCase) that was classified into glycoside hydrolase family 12 has been found to be effectively expressed in the industrial strain Pichia pastoris. Here, recombinant FI-CMCase was crystallized. Crystals belonging to the orthorhombic space group C222(1), with unit-cell parameters a = 74.2, b = 75.1, c = 188.4 angstrom, were obtained by the sitting-drop vapour-diffusion method and diffracted to 1.6 angstrom resolution. Initial phase determination by molecular replacement clearly shows that the crystal contains two protein molecules in the asymmetric unit. Further model building and structure refinement are in progress.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
关键词[WOS]	CELLULASE FI-CMCASE ; PICHIA-PASTORIS ; SACCHAROMYCES-CEREVISIAE ; PROTEIN-PRODUCTION ; ESCHERICHIA-COLI ; RATIONAL DESIGN ; EXPRESSION ; ENDOGLUCANASE ; PURIFICATION ; INHIBITION
收录类别	SCI
语种	英语
WOS记录号	WOS:000352508000006
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1450]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Peoples R China 2.Genozyme Biotechnol Inc, Taipei 106, Taiwan 3.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yun,Huang, Jian-Wen,Chen, Chun-Chi,et al. Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-beta-D-glucanase from Aspergillus aculeatus F-50[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2015,71(71):397-400.
APA	Chen, Yun,Huang, Jian-Wen,Chen, Chun-Chi,Lai, Hui-Lin,Jin, Jian,&Guo, Rey-Ting.(2015).Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-beta-D-glucanase from Aspergillus aculeatus F-50.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,71(71),397-400.
MLA	Chen, Yun,et al."Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-beta-D-glucanase from Aspergillus aculeatus F-50".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 71.71(2015):397-400.