Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5

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	Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5
	Men, Yan 1; Zhu, Yueming 1; Zhang, Lili 1; Kang, Zhenkui 2; Izumori, Ken 1; Sun, Yuanxia 1; Ma, Yanhe 1
刊名	MICROBIOLOGICAL RESEARCH
	2014
卷号	169 期号:2-3 页码:171-178
关键词	L-Arabinose isomerase D-Tagatose Food-grade microorganisms Pediococcus pentosaceus
英文摘要	The gene encoding L-arabinose isomerase from food-grade strain Pediococcus pentosaceus PC-5 was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and characterized. It was optimally active at 50 degrees C and pH 6.0. Furthermore, this enzyme exhibited a weak requirement for metallic ions for its maximal activity evaluated at 0.6 mM Mn2+ or 0.8 mM Co2+. Interestingly, this enzyme was distinguished from other L-AIs, it could not use L-arabinose as its substrate. In addition, a three-dimensional structure of L.-AI was built by homology modeling and L-arabinose and D-galactose were docked into the active site pocket of PPAI model to explain the interaction between L-AI and its substrate. The purified P. pentosaceus PC-5 L-AI converted P-galactose into D-tagatose with a high conversion rate of 52% after 24 h at 50 degrees C, suggesting its excellent potential in D-tagatose production. Crown Copyright (C) 2013 Published by Elsevier GmbH. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	THERMAL-STABILITY ; GLUCOSE ISOMERASE ; PROTEIN-STRUCTURE ; ESCHERICHIA-COLI ; SWISS-MODEL ; PH OPTIMUM ; ACIDIC PH ; PURIFICATION ; EXPRESSION ; STRAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000330335600008
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1409]
专题	天津工业生物技术研究所_功能糖与天然活性物质研究组孙媛霞 _期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Shanxi Tianjiao Biol Co Ltd, Shanxin 030006, Peoples R China
推荐引用方式 GB/T 7714	Men, Yan,Zhu, Yueming,Zhang, Lili,et al. Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5[J]. MICROBIOLOGICAL RESEARCH,2014,169(2-3):171-178.
APA	Men, Yan.,Zhu, Yueming.,Zhang, Lili.,Kang, Zhenkui.,Izumori, Ken.,...&Ma, Yanhe.(2014).Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5.MICROBIOLOGICAL RESEARCH,169(2-3),171-178.
MLA	Men, Yan,et al."Enzymatic conversion of D-galactose to D-tagatose: Cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC-5".MICROBIOLOGICAL RESEARCH 169.2-3(2014):171-178.