AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21
	Gongpan, Pianchou 1,2; Lu, Yanting 1,2; Wang, Fang 1,2; Xu, Yuhui 1,2; Xiong, Wenyong1
刊名	CELL CYCLE
	2016
卷号	15 期号:13 页码:1733-1741
关键词	AS160/TBC1D4 cell cycle cell proliferation G1/S p21
英文摘要	AS160 (TBC1D4) has been implicated in multiple biological processes. However, the role and the mechanism of action of AS160 in the regulation of cell proliferation remain unclear. In this study, we demonstrated that AS160 knockdown led to blunted cell proliferation in multiple cell types, including fibroblasts and cancer cells. The results of cell cycle analysis showed that these cells were arrested in the G1 phase. Intriguingly, this inhibition of cell proliferation and the cell cycle arrest caused by AS160 depletion were glucose independent. Moreover, AS160 silencing led to a marked upregulation of the expression of the cyclin-dependent kinase inhibitor p21. Furthermore, whereas AS160 overexpression resulted in p21 downregulation and rescued the arrested cell cycle in AS160-depeleted cells, p21 silencing rescued the inhibited cell cycle and proliferation in the cells. Thus, our results demonstrated that AS160 regulates glucose-independent eukaryotic cell proliferation through p21-dependent control of the cell cycle, and thereby revealed a molecular mechanism of AS160 modulation of cell cycle and proliferation that is of general physiological significance.
类目[WOS]	Cell Biology
研究领域[WOS]	Cell Biology
关键词[WOS]	PANCREATIC BETA-CELLS ; AKT SUBSTRATE ; GLUT4 TRANSLOCATION ; INSULIN-RESISTANCE ; GLUCOSE-TRANSPORT ; SKELETAL-MUSCLE ; PLASMA-MEMBRANE ; PROTEIN AS160 ; TRAFFICKING ; MUTATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000380132600015
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/28516]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gongpan, Pianchou,Lu, Yanting,Wang, Fang,et al. AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21[J]. CELL CYCLE,2016,15(13):1733-1741.
APA	Gongpan, Pianchou,Lu, Yanting,Wang, Fang,Xu, Yuhui,&Xiong, Wenyong.(2016).AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21.CELL CYCLE,15(13),1733-1741.
MLA	Gongpan, Pianchou,et al."AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21".CELL CYCLE 15.13(2016):1733-1741.