Heating and temperature gradients of lipid bilayer samples induced by RF irradiation in MAS solid-state NMR experiments

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Heating and temperature gradients of lipid bilayer samples induced by RF irradiation in MAS solid-state NMR experiments
	Wang, Jing 1,2; Zhang, Zhengfeng 1; Zhao, Weijing 1; Wang, Liying 1; Yang, Jun 1
刊名	MAGNETIC RESONANCE IN CHEMISTRY
	2016-09-01
卷号	54 期号:9 页码:753-759
关键词	solid-state NMR MAS sample heating lipid bilayers RF field hydration salt concentration
英文摘要	The MAS solid-state NMR has been a powerful technique for studying membrane proteins within the native-like lipid bilayer environment. In general, RF irradiation in MAS NMR experiments can heat and potentially destroy expensive membrane protein samples. However, under practical MAS NMR experimental conditions, detailed characterization of RF heating effect of lipid bilayer samples is still lacking. Herein, using H-1 chemical shift of water for temperature calibration, we systematically study the dependence of RF heating on hydration levels and salt concentrations of three lipids in MAS NMR experiments. Under practical H-1 decoupling conditions used in biological MAS NMR experiments, three lipids show different dependence of RF heating on hydration levels as well as salt concentrations, which are closely associated with the properties of lipids. The maximum temperature elevation of about 10 degrees C is similar for the three lipids containing 200% hydration, which is much lower than that in static solid-state NMR experiments. The RF heating due to salt is observed to be less than that due to hydration, with a maximum temperature elevation of less than 4 degrees C in the hydrated samples containing 120 mmol l(-1) of salt. Upon RF irradiation, the temperature gradient across the sample is observed to be greatly increased up to 20 degrees C, as demonstrated by the remarkable broadening of H-1 signal of water. Based on detailed characterization of RF heating effect, we demonstrate that RF heating and temperature gradient can be significantly reduced by decreasing the hydration levels of lipid bilayer samples from 200% to 30%. Copyright (C) 2016 John Wiley & Sons, Ltd.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Chemistry, Physical ; Spectroscopy
研究领域[WOS]	Chemistry ; Spectroscopy
关键词[WOS]	ANGLE-SPINNING NMR ; CHEMICAL-SHIFT THERMOMETER ; PHOSPHOLIPID-BILAYERS ; MAGNETIC-RESONANCE ; CROSS-POLARIZATION ; BIOLOGICAL SAMPLES ; MEMBRANE-PROTEINS ; LEAD NITRATE ; GEL-PHASE ; SPECTROSCOPY
收录类别	SCI
语种	英语
WOS记录号	WOS:000382969800009
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9506]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jing,Zhang, Zhengfeng,Zhao, Weijing,et al. Heating and temperature gradients of lipid bilayer samples induced by RF irradiation in MAS solid-state NMR experiments[J]. MAGNETIC RESONANCE IN CHEMISTRY,2016,54(9):753-759.
APA	Wang, Jing,Zhang, Zhengfeng,Zhao, Weijing,Wang, Liying,&Yang, Jun.(2016).Heating and temperature gradients of lipid bilayer samples induced by RF irradiation in MAS solid-state NMR experiments.MAGNETIC RESONANCE IN CHEMISTRY,54(9),753-759.
MLA	Wang, Jing,et al."Heating and temperature gradients of lipid bilayer samples induced by RF irradiation in MAS solid-state NMR experiments".MAGNETIC RESONANCE IN CHEMISTRY 54.9(2016):753-759.