Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR

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	Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR
	Wang, Jun 1; Gui, Lang 2; Chen, Zong-Yan 1; Zhang, Qi-Ya 1
刊名	VIRUS GENES
	2016-08-01
卷号	52 期号:4 页码:484-494
关键词	G protein-coupled receptor (GPCR) Crucian carp herpesvirus (CaHV) C-terminal Subcellular localization N-myristoylation site
ISSN号	0920-8569
英文摘要	G protein-coupled receptors (GPCRs) are known as seven transmembrane domain receptors and consequently can mediate diverse biological functions via regulation of their subcellular localization. Crucian carp herpesvirus (CaHV) was recently isolated from infected fish with acute gill hemorrhage. CaHV GPCR of 349 amino acids (aa) was identified based on amino acid identity. A series of variants with truncation/deletion/substitution mutation in the C-terminal (aa 315-349) were constructed and expressed in fathead minnow (FHM) cells. The roles of three key C-terminal regions in subcellular localization of CaHV GPCR were determined. Lysine-315 (K-315) directed the aggregation of the protein preferentially at the nuclear side. Predicted N-myristoylation site (GGGWTR, aa 335-340) was responsible for punctate distribution in periplasm or throughout the cytoplasm. Predicted phosphorylation site (SSR, aa 327-329) and GGGWTR together determined the punctate distribution in cytoplasm. Detection of organelles localization by specific markers showed that the protein retaining K-315 colocalized with the Golgi apparatus. These experiments provided first evidence that different mutations of CaHV GPCR C-terminals have different affects on the subcellular localization of fish herpesvirus-encoded GPCRs. The study provided valuable information and new insights into the precise interactions between herpesvirus and fish cells, and could also provide useful targets for antiviral agents in aquaculture.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Genetics & Heredity ; Virology
研究领域[WOS]	Genetics & Heredity ; Virology
关键词[WOS]	PROTEIN-COUPLED RECEPTORS ; FLOUNDER PARALICHTHYS-OLIVACEUS ; AQUACULTURE ANIMALS ; VIRUS ; DISEASE ; CELLS ; IDENTIFICATION ; TRAFFICKING ; EXPRESSION ; INFECTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000382636200006
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28346]
专题	水生生物研究所_藻类生物学及应用研究中心_学位论文
作者单位	1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Shanghai Ocean Univ, Fisheries & Life Sci, Shanghai 200120, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jun,Gui, Lang,Chen, Zong-Yan,et al. Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR[J]. VIRUS GENES,2016,52(4):484-494.
APA	Wang, Jun,Gui, Lang,Chen, Zong-Yan,&Zhang, Qi-Ya.(2016).Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR.VIRUS GENES,52(4),484-494.
MLA	Wang, Jun,et al."Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR".VIRUS GENES 52.4(2016):484-494.