Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella)

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	Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella)
	Xu, Jing 1; Wu, Pei 1,2,3; Jiang, Wei-Dan 1,2,3; Liu, Yang 1,2,3; Jiang, Jun 1,2,3; Kuang, Sheng-Yao 4; Tang, Ling 4; Tang, Wu-Neng 4; Zhang, Yong-An 5; Zhou, Xiao-Qiu 1,2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2016-08-01
卷号	55 页码:64-87
关键词	Protein Grass carp (Ctenopharyngodon idella) Disease resistance Intestinal immune Tight junction Apoptosis Antioxidant
ISSN号	1050-4648
英文摘要	This study investigated the effects of dietary proteins on the growth, disease resistance, intestinal immune and physical barrier functions of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (264.11 +/- 0.76 g) were fed six diets containing graded levels of protein (143.1, 176.7, 217.2, 257.5, 292.2 and 322.8 g digestible protein kg(-1) diet) for 8 weeks. After the growth trial, fish were challenged with Aeromonas hydrophila and mortalities were recorded for 14 days. The results indicated that optimal dietary protein levels: increased the production of antibacterial components, up-regulated anti-inflammatory cytokines, inhibitor of kappa B alpha, target of rapamycin and ribosomal protein S6 kinases 1 mRNA levels, whereas down-regulated pro-inflammatory cytokines, nuclear factor kappa B (NF-kappa B) P65, NF-kappa B P52, c-Rel, I kappa B kinase I kappa B kinase gamma and eIF4E-binding proteins 2 mRNA levels in three intestinal segments of young grass carp (P < 0:05), suggesting that optimal dietary protein level could enhance fish intestinal immune barrier function; up-regulated the mRNA levels of tight junction complexes, B-cell lymphoma protein-2, inhibitor of apoptosis proteins, myeloid cell leukemia-1 and NF-E2-related factor 2, and increased the activities and mRNA levels of antioxidant enzymes, whereas down-regulated myosin light chain kinase, cysteinyl aspartic acid-protease 2, 3, 7, 8, 9, fatty acid synthetase ligand, apoptotic protease activating factor-1, Bcl-2 associated X protein, p38 mitogen-activated protein kinase, c-Jun N-terminal protein kinase and Kelch-like-ECH-associated protein 1b mRNA levels, and decreased reactive oxygen species, malondialdehyde and protein carbonyl contents in three intestinal segments of young grass carp (P < 0.05), indicating that optimal dietary protein level could improve fish intestinal physical barrier function. Finally, the optimal dietary protein levels for the growth performance (PWG) and against enteritis morbidity of young grass carp were estimated to be 286.82 g kg(-1) diet (250.66 g digestible protein kg(-1) diet) and 292.10 g kg(-1) diet (255.47 g digestible protein kg(-1) diet), respectively. (C) 2016 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	NF-KAPPA-B ; AMINO-ACID-METABOLISM ; BREAM SPARUS-AURATA ; SALMON SALMO-SALAR ; GENE-EXPRESSION ; OXIDATIVE STRESS ; JUVENILE JIAN ; VAR. JIAN ; AEROMONAS-HYDROPHILA ; ANTIOXIDANT STATUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000381537000007
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28342]
专题	水生生物研究所_藻类生物学及应用研究中心_学位论文
作者单位	1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Xu, Jing,Wu, Pei,Jiang, Wei-Dan,et al. Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:64-87.
APA	Xu, Jing.,Wu, Pei.,Jiang, Wei-Dan.,Liu, Yang.,Jiang, Jun.,...&Feng, Lin.(2016).Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,55,64-87.
MLA	Xu, Jing,et al."Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 55(2016):64-87.