Engineering Clostridium acetobutylicum for alcohol production

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	Engineering Clostridium acetobutylicum for alcohol production
	Hou, Xiaohu 1,2; Peng, Wanfeng 2; Xiong, Lian 2; Huang, Chao 2; Chen, Xuefang 2; Chen, Xinde2 ; Zhang, Weiguo 1
刊名	journal of biotechnology
	2013-06-20
卷号	166 期号:1-2 页码:25-33
关键词	C. acetobutylicum Alcohol production adc gene inactivation gshAB Expression cassette (EC) Sol operon
英文摘要	while clostridium acetobutylicum has been used for large-scale butanol production (abe fermentation), its by-product acetone cannot be used as a biofuel. in this study, c. acetobutylicum was engineered for alcohol titers (butanol plus ethanol). the adc gene was inactivated to eliminate acetone production, and glutathione biosynthetic capability was introduced into c. acetobutylicum to improve the strain's robustness by expressing escherichia coli's gshab genes in the adc locus. acetone production was reduced from 2.64 +/- 0.22 g/l to 0.15 +/- 0.08 g/l in the engineered strain 824adc::gsh, whereas butanol production was increased from 5.17 +/- 0.26 g/l to 8.27 +/- 0.27 g/l. to further improve the alcohol titers, the metabolic flux in the alcohol biosynthesis pathways was enhanced. overlapping pcr was used to generate expression cassette ec, which expresses the hbd, thl, crt, and bcd genes, and the sol operon was amplified to express the adhe and ctfab genes. butanol and alcohol production reached 14.86 +/- 0.26 g/l and 18.11 +/- 0.66 g/l, respectively, in 824adc:: gsh sol-ec. furthermore, the butanol and alcohol yields were 0.336 g/g and 0.409 g/g, respectively, in 824adc:: gsh sol-ec. this study provided a combined strategy for enhancing alcohol production in c. acetobutylicum. (c) 2013 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	fermentative butanol production ; down-regulation ; atcc 824 ; transcriptional regulation ; molecular characterization ; gene ; disruption ; expression ; butyrate ; thiolase
收录类别	SCI
语种	英语
WOS记录号	WOS:000320651500005
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/10131]
专题	中国科学院广州能源研究所集成技术研发中心
作者单位	1.JiangNan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Hou, Xiaohu,Peng, Wanfeng,Xiong, Lian,et al. Engineering Clostridium acetobutylicum for alcohol production[J]. journal of biotechnology,2013,166(1-2):25-33.
APA	Hou, Xiaohu.,Peng, Wanfeng.,Xiong, Lian.,Huang, Chao.,Chen, Xuefang.,...&Zhang, Weiguo.(2013).Engineering Clostridium acetobutylicum for alcohol production.journal of biotechnology,166(1-2),25-33.
MLA	Hou, Xiaohu,et al."Engineering Clostridium acetobutylicum for alcohol production".journal of biotechnology 166.1-2(2013):25-33.