Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri

	Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri
	Miao, Guoying; Qi, Haigang; Li, Li; Que, Huayong; Zhang, Guofan
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2016-07-01
卷号	60 页码:1-11
关键词	Zhikong scallop Chlamys farreri Inhibitor of apoptosis Apoptosis Innate immunity
英文摘要	The proteins of inhibitor of apoptosis (IAP) family play important roles in regulation of apoptosis, immunological response and cell proliferation. Here we reported two IAP genes (named CfIAP1 and CfIAP2) in Zhikong scallop Chlamys farreri. The full-length CfIAP1 cDNA contained 1552 nucleotides, encoding a predicted protein of 251 amino acids with two BIR domains. The full-length CfIAP2 cDNA contained 1243 nt, encoding a 356-aa protein with one BIR domain and one RING domain. The two genes are ubiquitously expressed in six types of tissue of C. farreri. The expression levels of CfIAP1 and CfIAP2 were significantly up-regulated after challenged with acute viral necrobiotic disease virus, lipopolysaccharide and exposure to air. Subcellular localization assay showed that CfIAP1 was mainly distributed in cytoplasm and CfIAP2 was in cytoplasm and nucleus. As assessed using a kit designed to test Caspase3 function in mammalian cells, the activity of CfCaspase3 was enhanced as a result of the down-regulation of CfIAP2 expression by dsRNA-mediated gene silencing. Our study indicated that CfIAP1 and CfIAP2 may participate in the innate immunity and stress responses and that CfIAP2 might block apoptosis via inhibiting CfCaspase3 indirectly through an unexplored mechanism in C. farreri. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000374613800001
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/131016]
专题	海洋研究所_海洋生物技术研发中心
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Miao, Guoying,Qi, Haigang,Li, Li,et al. Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,60:1-11.
APA	Miao, Guoying,Qi, Haigang,Li, Li,Que, Huayong,&Zhang, Guofan.(2016).Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,60,1-11.
MLA	Miao, Guoying,et al."Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 60(2016):1-11.