Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite

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	Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite
	Chen, Rongxiang 1,2; Deng, Yinghua 3; Yang, Liu 3; Wang, Jie 2; Xu, Fuqiang 2,4
刊名	JOURNAL OF CHROMATOGRAPHIC SCIENCE
	2016-04-01
卷号	54 期号:4 页码:547-553
英文摘要	A fast and sensitive method was developed for in vivo determination of histamine in the brain microdialysate by reverse ion pair chromatography with electrochemical detection. The microdialysates were derivatized with o-phthalaldehyde and sodium sulfite, and separation was achieved using isocratic elution within 10 min. The separation was performed in an Agilent Eclipse Plus C18 column (3.0 x 150 mm, particle size 3.5 mu m), and the mobile phase consisted of 100 mM monosodium phosphate (pH 6.0), 500 mg L-1 OSA and 20% methanol (v/v). The linearity (R-2) was found to be >0.999, with a range from 2 to 50 nM and excellent repeatability (relative standard deviation, 2.29-6.04%), and the limit of detection was 0.4 nM. This method was successfully applied to analyze the extracellular concentration of histamine in the hypothalamus of rats, with probe recovery calculated in vivo.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	ELECTROCHEMICAL DETECTION ; FLUORESCENCE DETECTION ; MICRODIALYSIS SAMPLES ; BIOLOGICAL SAMPLES ; BRAIN HISTAMINE ; RAT-BRAIN ; IN-VITRO ; NEUROTRANSMITTERS ; QUANTITATION ; GLUTAMATE
收录类别	SCI
语种	英语
WOS记录号	WOS:000380263900009
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9479]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Zunyi Med Univ, Res Ctr Med & Biol, Zunyi 563003, Guizhou, Peoples R China 2.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China 3.Hubei Univ Educ, Dept Chem & Life Sci, Wuhan 430205, Hubei, Peoples R China 4.Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Chen, Rongxiang,Deng, Yinghua,Yang, Liu,et al. Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016,54(4):547-553.
APA	Chen, Rongxiang,Deng, Yinghua,Yang, Liu,Wang, Jie,&Xu, Fuqiang.(2016).Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite.JOURNAL OF CHROMATOGRAPHIC SCIENCE,54(4),547-553.
MLA	Chen, Rongxiang,et al."Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite".JOURNAL OF CHROMATOGRAPHIC SCIENCE 54.4(2016):547-553.