Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 污染过程与控制实验室

	Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa
	Cong, M; Zhao, JM; Lu, JS ; Ren, ZM ; Wu, HF
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2016-09-01
卷号	34 期号:5 页码:1034-1043
关键词	Suaeda salsa halophyte phytochelatin synthase (PCS) homologous cloning heavy metal tissue distribution
ISSN号	0254-4059
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
产权排序	[Cong Ming; Zhao Jianmin; Wu Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Lu Jiasen; Ren Zhiming] Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
英文摘要	The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity. Since phytochelatins can effectively chelate heavy metals, it was hypothesized that S. salsa possessed a phytochelatin synthase (PCS) gene. In the present study, the cDNA of PCS was obtained from S. salsa (designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends (RACE). A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides, encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain. A similarity analysis suggested that SsPCS shared up to a 58.6% identity with other PCS proteins and clustered with PCS proteins from eudicots. There was a new kind of metal ion sensor motif in its C-terminal domain. The SsPCS transcript was more highly expressed in elongated and fibered roots and stems (P < 0.05) than in leaves. Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS (P < 0.05). To the best of our knowledge, SsPCS is the second PCS gene cloned from a halophyte, and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila. This study provided a new view of halophyte PCS genes in heavy metal tolerance.
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	HEAVY-METALS ; DUNALIELLA-TERTIOLECTA ; CADMIUM EXPOSURE ; EISENIA-FOETIDA ; ARABIDOPSIS ; PROTEIN ; ACCUMULATION ; RESPONSES ; DOMAIN ; PLANTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000379737000015
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/17108]
专题	烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc 2.Yantai Univ, Sch Biol
推荐引用方式 GB/T 7714	Cong, M,Zhao, JM,Lu, JS,et al. Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(5):1034-1043.
APA	Cong, M,Zhao, JM,Lu, JS,Ren, ZM,&Wu, HF.(2016).Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(5),1034-1043.
MLA	Cong, M,et al."Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.5(2016):1034-1043.