Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host

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	Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host
	Ou, Tong; Gao, Xiao-Chan; Li, San-Hua; Zhang, Qi-Ya
刊名	JOURNAL OF GENERAL VIROLOGY
	2015-12-01
卷号	96 页码:3681-3697
ISSN号	0022-1317
英文摘要	The genome sequence, genetic characterization and nblA gene function of Microcystis aeruginosa myovirus isolated from Lake Dianchi in China (MaMV-DC) have been analysed. The genome DNA is 169 223 bp long, with 170 predicted protein-coding genes (001L-170L) and a tRNA gene. About one-sixth of these genes have homologues in the host cyanobacteria M. aeruginosa. The genome carries a gene homologous to host nblA, which encodes a protein involved in the degradation of cyanobacterial phycobilisome. Its expression during MaMV-DC infection was confirmed by reverse transcriptase PCR and Western blot detection and abundant expression was companied by the significant decline of phycocyanin content and massive release of progeny MaMV-DC. In addition, expressing MaMV-DC nblA reduced the phycocyanin peak and the phycocyanin to chlorophyll ratio in model cyanobacteria. These results confirm that horizontal gene transfer events have occurred between cyanobacterial host and cyanomyovirus and suggest that MaMV-DC carrying host-derived genes (such as 005L, that codes for NblA) is responsible for more efficient expression of cyanophage genes and release of progeny cyanophage. This study provides novel insight into the horizontal gene transfer in cyanophage and the interactions between cyanophage and their host.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Virology
关键词[WOS]	MARINE SYNECHOCOCCUS STRAINS ; PHYCOBILISOME DEGRADATION ; PHOTOSYNTHESIS GENES ; ACQUIRED-RESISTANCE ; CYANOPHAGE ; SEQUENCE ; PHAGES ; PROCHLOROCOCCUS ; DIVERSITY ; VIRUSES
收录类别	SCI
语种	英语
WOS记录号	WOS:000369521800024
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27707]
专题	水生生物研究所_水生生物多样性与资源保护研究中心
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Ou, Tong,Gao, Xiao-Chan,Li, San-Hua,et al. Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host[J]. JOURNAL OF GENERAL VIROLOGY,2015,96:3681-3697.
APA	Ou, Tong,Gao, Xiao-Chan,Li, San-Hua,&Zhang, Qi-Ya.(2015).Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host.JOURNAL OF GENERAL VIROLOGY,96,3681-3697.
MLA	Ou, Tong,et al."Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host".JOURNAL OF GENERAL VIROLOGY 96(2015):3681-3697.