Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell

	Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell
	Wang M(王萌); Feng WY(丰伟悦); Li B(李柏); Wang B(汪冰); Zhu MT(朱墨桃); Wang Y(王云); Yuan H(袁慧); Zhao YL(赵宇亮); Chai ZF(柴之芳); Wang, M
刊名	ANALYTICAL CHEMISTRY
	2007
卷号	79 期号:23 页码:9128-9134
通讯作者	Chinese Acad Sci, Inst High Energy Phys, Lab Bio Environm Effects Nanomat & Nanosafely, Key Lab Nucl Anal Tech, Beijing, Peoples R China ; Chinese Acad Sci, Grad Sch, Beijing, Peoples R China ; Shanghai Jiao Tong Univ, Shanghai, Peoples R China
英文摘要	Quantitative analysis of proteins is an essential part and also constitutes a major challenge in modem proteomics. Quantification of proteins by inductively coupled plasma mass spectrometry (ICPMS) offers an alternative method for quantitative proteomics. In this study, we developed a method of absolute quantification of proteins via sulfur by size exclusion chromatography (SEC) coupled to ICPMS with a collision cell (ICP-CC-MS) and postcolumn isotope dilution. Bovine serum albumin (BSA), superoxide dismutase (SOD), and metallothionein-II (MT-II) served as model proteins. Enriched (34)S, (65)Cu, and (67)Zn isotopic solutions were continuously mixed with the eluate from the SEC. Oxygen was added as a reactive gas into the collision cell where sulfur reacts with oxygen to form sulfur-oxygen ion, the ratio of (32)S(16)O(+)/(34)S(16)O(+) thus representing (32)S(+)/(34)S(+). The absolute quantity of proteins could be calculated by the isotopic dilution equation and the content of sulfur in the proteins. The detection limits for BSA, SOD, and MT-II are 8, 31, and 15 pmol, respectively. The relative standard deviations for the proteins are less than 3%. The ratios of S/Cu and S/Zn in the proteins were also determined. The quantitative method was validated by comparing with gravimetric results.
学科主题	Chemistry
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
原文出处	SCI
语种	英语
WOS记录号	WOS:000251311900045
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/240156]
专题	高能物理研究所_院士高能物理研究所_实验物理中心高能物理研究所_加速器中心高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Wang M,Feng WY,Li B,et al. Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell[J]. ANALYTICAL CHEMISTRY,2007,79(23):9128-9134.
APA	王萌.,丰伟悦.,李柏.,汪冰.,朱墨桃.,...&Chai, ZF.(2007).Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell.ANALYTICAL CHEMISTRY,79(23),9128-9134.
MLA	王萌,et al."Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell".ANALYTICAL CHEMISTRY 79.23(2007):9128-9134.