A large-scale, high-efficiency and low-cost platform for structural genomics studies

	A large-scale, high-efficiency and low-cost platform for structural genomics studies
	Su, XD; Liang, YH; Li, LF; Nan, J; Brostromer, E; Liu, P; Dong, YH; Xian, DC; Liu P(刘鹏); Dong YH(董宇辉)
刊名	ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
	2006
卷号	62 期号:8 页码:843-851
通讯作者	Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China
英文摘要	A large- scale, high- efficiency and low- cost platform based on a Beckman Coulter Biomek FX and custom- made automation systems for structural genomics has been set up at Peking University, Beijing, People's Republic of China. This platform has the capacity to process up to 2000 genes per year for structural and functional analyses. Bacillus subtilis, a model organism for Gram- positive bacteria, and Streptococcus mutans, a major pathogen of dental caries, were selected as the main targets. To date, more than 470 B. subtilis and 1200 S. mutans proteins and hundreds of proteins from other sources, including human liver proteins, have been selected as targets for this platform. The selected genes are mainly related to important metabolism pathways and/ or have potential relevance for drug design. To date, 40 independent structures have been determined; of these 11 are in the category of novel structures by the criterion of having less than 30% sequence identity to known structures. More than 13 structures were determined by SAD/ MAD phasing. The macromolecular crystallography beamline at the Beijing Synchrotron Radiation Facility and modern phasing programs have been crucial components of the operation of the platform. The idea and practice of the genomic approach have been successfully adopted in a moderately funded structural biology program and it is believed this adaptation will greatly improve the production of protein structures. The goal is to be able to solve a protein structure of moderate difficulty at a cost about US $ 10 000.
学科主题	Biochemistry & Molecular Biology; Biophysics; Crystallography
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
原文出处	SCI
语种	英语
WOS记录号	WOS:000239119800001
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/238594]
专题	高能物理研究所_院士
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Su, XD,Liang, YH,Li, LF,et al. A large-scale, high-efficiency and low-cost platform for structural genomics studies[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2006,62(8):843-851.
APA	Su, XD.,Liang, YH.,Li, LF.,Nan, J.,Brostromer, E.,...&冼鼎昌.(2006).A large-scale, high-efficiency and low-cost platform for structural genomics studies.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,62(8),843-851.
MLA	Su, XD,et al."A large-scale, high-efficiency and low-cost platform for structural genomics studies".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 62.8(2006):843-851.