Solution structure of Kti11p from Saccharomyces cerevisiae reveals a novel zinc-binding module

	Solution structure of Kti11p from Saccharomyces cerevisiae reveals a novel zinc-binding module
	Sun, JP; Zhang, JH; Wu, FM; Xu, C; Li, SJ; Zhao, W; Wu, ZY; Wu, JH; Zhou, CZ; Shi, YY
刊名	BIOCHEMISTRY
	2005
卷号	44 期号:24 页码:8801-8809
通讯作者	Univ Sci & Technol China, Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China ; Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China ; Chinese Acad Sci, Beijing Synchrotron Radiat Facil, Inst High Energy Phys, Beijing 100039, Peoples R China
英文摘要	Kti11p is a small, highly conserved CSL zinc finger-containing protein found in many eukaryotes. It was first identified as one of the factors required for maintaining the sensitivity of Saccharomyces cerevisiae to Kluyveromyces lactis zymocin. Then, it was found to be identical to Dph3, a protein required for diphthamide biosynthesis on eEF-2, the target of diphtheria toxin and Pseudomonas exotoxin A, in both yeast and higher eukaryotes. Furthermore, Kti11p/Dph3 was found to physically interact with core-Elongator, ribosomal proteins, eEF-2, two other proteins required for diphthamide modification on eEF-2, and De1GEF. Here, we determined the solution structure of Kti11p using NMR, providing the first structure of the CSL-class zinc-binding protein family. We present the first experimental evidence that Kti11p can bind a single Zn2+ ion by its four conserved cysteine residues. The major structure of Kti11p comprises a beta sandwich as well as an a helix. Moreover, a structure-based similarity search suggests that it represents a novel structure and may define a new family of the zinc ribbon fold group. Therefore, our work provides a molecular basis for further understanding the multiple functions of Kti11p/Dph3 in different biological processes.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
原文出处	SCI
语种	英语
WOS记录号	WOS:000229994900025
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/240152]
专题	高能物理研究所_多学科研究中心高能物理研究所_实验物理中心高能物理研究所_加速器中心中国科学院高能物理研究所_大装置管理中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Sun, JP,Zhang, JH,Wu, FM,et al. Solution structure of Kti11p from Saccharomyces cerevisiae reveals a novel zinc-binding module[J]. BIOCHEMISTRY,2005,44(24):8801-8809.
APA	Sun, JP.,Zhang, JH.,Wu, FM.,Xu, C.,Li, SJ.,...&吴自玉.(2005).Solution structure of Kti11p from Saccharomyces cerevisiae reveals a novel zinc-binding module.BIOCHEMISTRY,44(24),8801-8809.
MLA	Sun, JP,et al."Solution structure of Kti11p from Saccharomyces cerevisiae reveals a novel zinc-binding module".BIOCHEMISTRY 44.24(2005):8801-8809.