Ectopic expression of Jatropha curcas APETALA1 (JcAP1) caused early flowering in Arabidopsis, but not in Jatropha

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 能源植物分子育种研究组

	Ectopic expression of Jatropha curcas APETALA1 (JcAP1) caused early flowering in Arabidopsis, but not in Jatropha
	Xu, Zeng-Fu
刊名	PEERJ
	2016
卷号	4 期号:X 页码:E1969
关键词	Jatropha Flower identity Arabidopsis APETALA1 Physic nut Flowering
中文摘要	Jatropha curcas is a promising feedstock for biofuel production because Jatropha oil is highly suitable for the production of biodiesel and bio-jet fuels. However, Jatropha exhibits a low seed yield as a result of unreliable and poor flowering. APETALA1 (AP1) is a floral meristem and organ identity gene in higher plants. The flower meristem identity genes of Jatropha have not yet been identified or characterized. To better understand the genetic control of flowering in Jatropha, an AP1 hornolog (JcAP1) was isolated from Jatropha. An amino acid sequence analysis of JcAP1 revealed a high similarity to the AP1 proteins of other perennial plants. JcAP1 was expressed in inflorescence buds, flower buds, sepals and petals. The highest expression level was observed during the early developmental stage of the flower buds. The overexpression of JcAP1 using the cauliflower mosaic virus (CaMV) 35S promoter resulted in extremely early flowering and abnormal flowers in transgenic Arabidopsis plants. Several flowering genes downstream of AP1 were up-regulated in the JcAP1-overexpressing transgenic plant lines. Furthermore, JcAP1 overexpression rescued the phenotype caused by the Arabidopsis AP1 loss-of-function mutant ap1-11. Therefore, JcAP1 is an ortholog of flowering in of AtAP1 which plays a similar role in the regulation Arabidopsis. However, the overexpression of JcAP1 in Jatropha using the same promoter resulted in little variation in the flowering time and floral organs, indicating that JcAP1 may be insufficient to regulate flowering by itself in Jatropha. This study helps to elucidate the function of JcAP1 and contributes to the understanding of the moecular mechanisms of flower development in Jatropha.
公开日期	2016-06-06
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/9880]
专题	西双版纳热带植物园_能源植物分子育种研究组
通讯作者	Xu, Zeng-Fu
推荐引用方式 GB/T 7714	Xu, Zeng-Fu. Ectopic expression of Jatropha curcas APETALA1 (JcAP1) caused early flowering in Arabidopsis, but not in Jatropha[J]. PEERJ,2016,4(X):E1969.
APA	Xu, Zeng-Fu.(2016).Ectopic expression of Jatropha curcas APETALA1 (JcAP1) caused early flowering in Arabidopsis, but not in Jatropha.PEERJ,4(X),E1969.
MLA	Xu, Zeng-Fu."Ectopic expression of Jatropha curcas APETALA1 (JcAP1) caused early flowering in Arabidopsis, but not in Jatropha".PEERJ 4.X(2016):E1969.