Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles

	Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles
	Hu,Jingting; Ni,Pengjuan; Dai,Haichao; Sun,Yujing; Wang,Yilin; Jiang,Shu; Li,Zhuang
刊名	analyst
	2015
卷号	140 期号:10 页码:3581-3586
关键词	PEROXIDASE-LIKE ACTIVITY TOXIC LECTINS RICIN SELECTION GLUCOSE POLYMERASE PARTICLES MECHANISM LIGANDS PROBES
通讯作者	li,z
英文摘要	in this study we propose a simple and sensitive colorimetric aptasensor for the quantitative analysis of abrin by using catalytic aunps for the first time. aunps possess the peroxidase-like activity that can catalyse 3,3,5,5-tetramethylbenzidine (tmb) in the presence of h2o2, leading to color change of the solution. it is interesting to find that the peroxidase-like activity of aunps can be improved by surface activation with a target-specific aptamer. however, with a target molecule, the aptamer is desorbed from the aunps surface, resulting in a decrease of the catalytic abilities of aunps. the color change of the solution is relevant to the target concentration, and this can be judged by the naked eye and monitored by using a uv-vis spectrometer. the linear range for the current analytical system was from 0.2 nm to 17.5 nm. the corresponding limit of detection (lod) was 0.05 nm. some other proteins such as thrombin (th), glucose oxidase (gox), and bovine serum albumin (bsa) all had a negligible effect on the determination of abrin. furthermore, several practical samples spiked with abrin were analyzed using the proposed method with excellent recoveries. this aptamer-based colorimetric biosensor is superior to other conventional methods owing to its simplicity, low cost, and high sensitivity.
收录类别	SCI
语种	英语
WOS记录号	WOS:000354192500036
公开日期	2016-05-31
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/65184]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Hu,Jingting,Ni,Pengjuan,Dai,Haichao,et al. Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles[J]. analyst,2015,140(10):3581-3586.
APA	Hu,Jingting.,Ni,Pengjuan.,Dai,Haichao.,Sun,Yujing.,Wang,Yilin.,...&Li,Zhuang.(2015).Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles.analyst,140(10),3581-3586.
MLA	Hu,Jingting,et al."Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles".analyst 140.10(2015):3581-3586.