Label-Free Surface-Enhanced Infrared Spectroelectrochemistry Studies the Interaction of Cytochrome c with Cardiolipin-Containing Membranes

	Label-Free Surface-Enhanced Infrared Spectroelectrochemistry Studies the Interaction of Cytochrome c with Cardiolipin-Containing Membranes
	Liu,Li; Zeng,Li; Wu,Lie; Jiang,Xiue
刊名	journal of physical chemistry c
	2015
卷号	119 期号:8 页码:3990-3999
关键词	ABSORPTION SPECTROSCOPY REORGANIZATION ENERGY PEROXIDASE-ACTIVITY MODIFIED ELECTRODES GOLD ELECTRODE HEME EXPOSURE PROTEIN REDUCTION WATER DYNAMICS
通讯作者	jiang,xe
英文摘要	interactions of cytochrome c (cyt c) with cardiolipin (cl)-containing membranes have been revealed to play an important role in inducing early apoptosis. in this paper, we studied the interaction of cyt c with solid-supported cl-containing lipid bilayers by a lable-free spectroelectrochemistry method. the biomimetic membrane was fabricated by fusing cardiolipin-phosphatidylcholine (cl_pc, 1:4) vesicles onto the hydrophobic surface of a preadsorbed 1-dodecanethiol (dt) on a gold electrode (cl_pc/dt/au). the adsorption of cyt c onto the cl_pc/dt/au was in-situ studied by cyclic voltammetry (cv) and surface-enhanced infrared adsorption spectroscopy (seiras). the electrochemistry of cyt c/cl_pc/dt/au exhibits the formal potential (e-f) at 0.21 v (vs ag/agcl), a positive shift of 190 mv as compared to that of native cyt c adsorbed at the mercaptoundecanoic acid-modified gold electrode (cyt c/mua/au). the potential-induced seira difference spectroscopy discloses that cyt c was loosely adsorbed onto cl_pc/dt/au in a different orientation and underwent a new beta-sheet formation relative to native cyt c adsorbed onto mua/au. it could be the enhanced hydration change of cl-bound cyt c upon oxidization and reduction and the different adsorption orientation that determine the positive shift in its formal potential.
收录类别	SCI
语种	英语
WOS记录号	WOS:000350329300013
公开日期	2016-05-31
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/65060]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Liu,Li,Zeng,Li,Wu,Lie,et al. Label-Free Surface-Enhanced Infrared Spectroelectrochemistry Studies the Interaction of Cytochrome c with Cardiolipin-Containing Membranes[J]. journal of physical chemistry c,2015,119(8):3990-3999.
APA	Liu,Li,Zeng,Li,Wu,Lie,&Jiang,Xiue.(2015).Label-Free Surface-Enhanced Infrared Spectroelectrochemistry Studies the Interaction of Cytochrome c with Cardiolipin-Containing Membranes.journal of physical chemistry c,119(8),3990-3999.
MLA	Liu,Li,et al."Label-Free Surface-Enhanced Infrared Spectroelectrochemistry Studies the Interaction of Cytochrome c with Cardiolipin-Containing Membranes".journal of physical chemistry c 119.8(2015):3990-3999.