Improved islet purity by the hypertonic-hypotonic method

	Improved islet purity by the hypertonic-hypotonic method
	Li, Na 1,2,3; Zhang, Ying 1; Liu, Yang 1; Xiu, Zhilong 2; Tan, Mingqian 1; Li, Shen 1,2; Guo, Xin 1; Wang, Yu 1,3; Chen, Li 1,3; Song, Yizhe 1,3
刊名	international journal of artificial organs
	2014-06-01
卷号	37 期号:6 页码:477-485
关键词	Islets isolation Purification Hypertonic-hypotonic method Plackett-Burman method Density gradient separation
通讯作者	张英
英文摘要	introduction: islet purification is usually performed using the density gradient separation method, but the purity of islets is low because exocrine cells and the embedded islets are hard to remove by using only the density gradient method. the aim of this study was to establish a new islet purification process comprising a hypertonic-hypotonic treatment step followed by a density gradient centrifugation step to improve the purity of islets.
学科主题	物理化学
WOS标题词	science & technology ; technology ; life sciences & biomedicine
类目[WOS]	engineering, biomedical ; transplantation
研究领域[WOS]	engineering ; transplantation
关键词[WOS]	cell transplantation ; pancreatic-islets ; purification ; optimization ; osmolality ; langerhans ; iodixanol ; survival ; density ; ficoll
收录类别	SCI
语种	英语
WOS记录号	WOS:000343850600006
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144518]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Na,Zhang, Ying,Liu, Yang,et al. Improved islet purity by the hypertonic-hypotonic method[J]. international journal of artificial organs,2014,37(6):477-485.
APA	Li, Na.,Zhang, Ying.,Liu, Yang.,Xiu, Zhilong.,Tan, Mingqian.,...&Ma, Xiaojun.(2014).Improved islet purity by the hypertonic-hypotonic method.international journal of artificial organs,37(6),477-485.
MLA	Li, Na,et al."Improved islet purity by the hypertonic-hypotonic method".international journal of artificial organs 37.6(2014):477-485.