Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry

	Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry
	Zhao, Jieyu; Hu, Chunxiu; Zeng, Jun; Zhao, Yanni; Zhang, Junjie; Chang, Yuwei; Li, Lili; Zhao, Chunxia; Lu, Xin; Xu, Guowang
刊名	metabolomics
	2014-10-01
卷号	10 期号:5 页码:805-815
关键词	Capillary electrophoresis Mass spectrometry Plant metabolomics Method validation Tobacco leaf
通讯作者	许国旺
英文摘要	many metabolites in plant are highly polar and ionic. their analysis using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry can be problematic. therefore a capillary electrophoresis-mass spectrometry (ce-ms) method with charge-driven separation characteristic was developed to investigate polar metabolites in tobacco. to obtain as many features as possible, extraction of polar metabolites was optimized by the design of experiments and evaluated by univariate statistics. method validation was carried out to evaluate the analytical characteristics including calibration curve, precision, sample stability and extraction reproducibility. the developed method was successfully applied in studying 30 tobacco leaves obtained from yunnan and guizhou provinces in china. a total of 154 polar metabolites were identified based on available database. multivariate pattern recognition clearly revealed the metabolic differences between the two geographic areas and 43 significantly different metabolites were defined by the non-parametric hypothesis test (mann-whitney u test) and false discovery rate. some key metabolites involved in photosynthesis such as ribulose 1,5-disphosphate, fructose 1,6-diphosphate, glycine, betaine, gaba and serine were found to be susceptible to environmental conditions. this study shows that the metabolic profiling based on ce-ms can clearly discriminate tobacco leaves of different geographical origins and understand the relationship between plant metabolites and their geographical origins.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	endocrinology & metabolism
研究领域[WOS]	endocrinology & metabolism
关键词[WOS]	solid-phase microextraction ; false discovery rate ; liquid-chromatography ; gas-chromatography ; ce-ms ; metabolomics ; extraction ; plant ; leaves ; optimization
收录类别	SCI
语种	英语
WOS记录号	WOS:000341422600004
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144497]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jieyu,Hu, Chunxiu,Zeng, Jun,et al. Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry[J]. metabolomics,2014,10(5):805-815.
APA	Zhao, Jieyu.,Hu, Chunxiu.,Zeng, Jun.,Zhao, Yanni.,Zhang, Junjie.,...&Xu, Guowang.(2014).Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry.metabolomics,10(5),805-815.
MLA	Zhao, Jieyu,et al."Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry".metabolomics 10.5(2014):805-815.