Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum

	Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum
	Liu, Jinxiang 1,2; Deng, Qiliang 1; Tao, Dingyin 1,2; Yang, Kaiguang 1; Zhang, Lihua 1; Liang, Zhen 1; Zhang, Yukui 1
刊名	scientific reports
	2014-06-30
卷号	4 期号:1 页码:1
通讯作者	张丽华
英文摘要	hierarchical imprinting was developed to prepare the protein imprinted materials, as the artificial antibody, for the selective depletion of hsa from the human serum proteome. porcine serum albumin (psa) was employed as the dummy template for the fabrication of the recognition sites. to demonstrate the advantages of the hierarchical imprinting, molecularly imprinted polymers prepared by hierarchical imprinting technique (h-mips) were compared with those obtained by bulk imprinting (b-mips), in terms of the binding capacity, adsorption kinetics, selectivity and synthesis reproducibility. the binding capacity of h-mips could reach 12 mg g(-1). and saturation binding could be reached in less than 20 min for the h-mips. in the protein mixture, h-mips exhibit excellent selectivity for psa, with imprinting factors as about 3.6, much higher than those for non-template proteins. for the proteomic application, the identified protein group number in serum treated by h-mips was increased to 422, which is 21% higher than that obtained from the original serum, meanwhile the identified protein group number for the albumin removal kit was only 376. the results demonstrate that protein imprinted polymers prepared by hierarchical imprinting technique, might become the artificial antibodies for the selective depletion of high abundance proteins in proteome study.
学科主题	物理化学
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	polymers ; nanoparticles ; recognition ; proteomics ; samples ; water
收录类别	SCI
语种	英语
WOS记录号	WOS:000338103700003
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144458]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jinxiang,Deng, Qiliang,Tao, Dingyin,et al. Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum[J]. scientific reports,2014,4(1):1.
APA	Liu, Jinxiang.,Deng, Qiliang.,Tao, Dingyin.,Yang, Kaiguang.,Zhang, Lihua.,...&Zhang, Yukui.(2014).Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum.scientific reports,4(1),1.
MLA	Liu, Jinxiang,et al."Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum".scientific reports 4.1(2014):1.