Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins

	Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins
	Zhang, Sitong 1,2; He, Xiwen 1,2; Chen, Langxing 1,2; Zhang, Yukui 1,2,3
刊名	new journal of chemistry
	2014-09-01
卷号	38 期号:9 页码:4212-4218
通讯作者	陈朗星
英文摘要	a simple, novel approach was developed for the preparation of boronic acid functionalized fe3o4 magnetic nanoparticles (mnps) via thiol-ene (te) click reaction. in this work, two clickable fe3o4 mnps functionalized with either alkene or thiol moieties were synthesized. firstly, fe3o4 mnps were synthesized through a solvothermal method and then the clickable alkene- or thiol-coated fe3o4 mnps were prepared by sot gel reaction with an organosilicon coupling agent, 3-(methacryloyloxy) propyltrimethoxylsilane (mps) or 3-mercaptopropyltriethoxysilane (mptes). the carbon carbon double bonds/thiol groups on the surface of the mnps serve as clickable sites to react with 4-mercaptophenylboronic acid (4-mpba)/3-acrylamidophenylboronic acid (aapba) during the subsequent te click reaction. finally, the high density of boronic acid ligands immobilized on the surface of the fe3o4 mnps was obtained via te click reactions. the morphology, adsorption and recognition properties of the fe3o4 mnps were investigated by transmission electron microscopy (tem), scanning electron microscopy (sem), x-ray diffraction (xrd), vibrating sample magnetomety (vsm) and x-ray photoelectron spectrometry (xps). four proteins, including ovalbumin (ob) and transferrin (trf) as glycoprotein templates, and lysozyme (lyz) and horse heart cytochrome c (cyt c) as non-glycoprotein templates are chosen as target proteins. two types of click-fe3o4 mnp (fe3o4@mps@pba and fe3o4@sh@aapba) exhibit a high binding capacity and excellent specificity towards glycoproteins, and can selectively capture and separate glycoproteins from egg white samples directly. furthermore, this work could provide a promising method of surface modification for the design of more efficient adsorbents for the isolation and enrichment of proteins from complex bio-samples.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	efficient strategy ; glycopeptides ; capture ; photopolymerization ; networks ; protein ; alkynes ; samples ; facile
收录类别	SCI
语种	英语
WOS记录号	WOS:000341065800024
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144442]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nankai Univ, Coll Chem, Res Ctr Analyt Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn, Tianjin, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Sitong,He, Xiwen,Chen, Langxing,et al. Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins[J]. new journal of chemistry,2014,38(9):4212-4218.
APA	Zhang, Sitong,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2014).Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins.new journal of chemistry,38(9),4212-4218.
MLA	Zhang, Sitong,et al."Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins".new journal of chemistry 38.9(2014):4212-4218.