Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures

	Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures
	Deng, Qiliang 1,2; Wu, Jianhua 2; Chen, Yang 1; Zhang, Zhijun 2; Wang, Yang 2; Fang, Guozhen 2; Wang, Shuo 2; Zhang, Yukui 3
刊名	journal of materials chemistry b
	2014-02-28
卷号	2 期号:8 页码:1048-1058
通讯作者	shuowang
英文摘要	phosphorylation of protein regulates nearly all biological processes in nature. the development of enrichment techniques for phosphorylated proteins is vital to systematic identification and characterization of phosphoproteins. here, a general strategy for highly efficient capture of intact phosphorylated proteins from protein mixtures has been developed by using guanidine functionalized superparamagnetic microspheres (denoted as fe3o4@sio2@gdn). the fe3o4@sio2@gdn was prepared by modifying fe3o4@sio2 with 3-guanidopropyl triethoxysilane as a functionalization monomer. the resulting materials could specifically and selectively recognize phosphoproteins and showed high binding capacities for model phosphoproteins (78.8 mg g(-1) for ovalbumin (ova) and 59.6 mg g(-1) for beta-casein (beta-cas), respectively). the feasibility of the resulting material for phosphoprotein enrichment has also been demonstrated by selectively binding and capturing phosphoproteins from complex protein mixtures and real samples (milk, egg, and tissue protein extract from a mouse liver), respectively. in addition, the selective enrichment of phosphopeptides has also been investigated. the proposed technique showed application potential for phosphoprotein and phosphopeptide enrichment.
学科主题	物理化学
WOS标题词	science & technology ; technology
类目[WOS]	materials science, biomaterials
研究领域[WOS]	materials science
关键词[WOS]	metal-affinity-chromatography ; phosphorylated proteins ; mass-spectrometry ; recognition ; purification ; strategies ; proteomics ; receptors ; mechanism
收录类别	SCI
语种	英语
WOS记录号	WOS:000331500800012
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144437]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Tianjin Univ Sci & Technol, Dept Sci, Tianjin 300457, Peoples R China 2.Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Deng, Qiliang,Wu, Jianhua,Chen, Yang,et al. Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures[J]. journal of materials chemistry b,2014,2(8):1048-1058.
APA	Deng, Qiliang.,Wu, Jianhua.,Chen, Yang.,Zhang, Zhijun.,Wang, Yang.,...&Zhang, Yukui.(2014).Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures.journal of materials chemistry b,2(8),1048-1058.
MLA	Deng, Qiliang,et al."Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures".journal of materials chemistry b 2.8(2014):1048-1058.