Preparative separation of nine flavonoids from Pericarpium Citri Reticulatae by preparative-HPLC and HSCCC

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	Preparative separation of nine flavonoids from Pericarpium Citri Reticulatae by preparative-HPLC and HSCCC
	Ye, Xiaolan ; Cao, Di ; Song, Fenyun ; Fan, Guorong ; Wu, Fuhai
刊名	SEPARATION SCIENCE AND TECHNOLOGY
	2016
卷号	51 期号:5 页码:807-815
关键词	POLYMETHOXYLATED FLAVONES ANTIINFLAMMATORY ACTIVITY LIQUID-CHROMATOGRAPHY IDENTIFICATION ANTICANCER CHEMISTRY APOPTOSIS EXTRACTS CELLS
ISSN号	0149-6395
中文摘要	An economical and systematized chromatographic separation process to isolate and purify flavonoids from Pericarpium Citri Reticulatae was developed. Solvent refluxing extraction, macroporous resin column, preparative HPLC and HSCCC (high-speed counter-current chromatography) were used for extraction, fraction, isolation, and purification of nine flavonoids including vicenin-2(42 mg), hesperidin (108 mg), isosinensetin (15 mg), sinensetin (10 mg), tetramethyl-O-isoscutellarein (11 mg), nobiletin (121 mg), 3, 5, 6, 7, 8, 3 ',4 '-heptamethoxyflavone (34 mg), tangeretin (114 mg), and 5-demethylnobiletin (13 mg) from Pericarpium Citri Reticulatae, and their purities were all above 98%. All these constituents were identified by UV, MS, H-1 NMR, and C-13 NMR. The procedure and techniques described here can provide a useful reference for the preparative-scale isolation of some flavonoids in a fast, cost-effective, and environmentally-friendly method.
学科主题	Chemistry ; Engineering
收录类别	SCI
原文出处	10.1080/01496395.2015.1122634
公开日期	2016-05-06
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/9740]
专题	西双版纳热带植物园_文献共享
推荐引用方式 GB/T 7714	Ye, Xiaolan,Cao, Di,Song, Fenyun,et al. Preparative separation of nine flavonoids from Pericarpium Citri Reticulatae by preparative-HPLC and HSCCC[J]. SEPARATION SCIENCE AND TECHNOLOGY,2016,51(5):807-815.
APA	Ye, Xiaolan,Cao, Di,Song, Fenyun,Fan, Guorong,&Wu, Fuhai.(2016).Preparative separation of nine flavonoids from Pericarpium Citri Reticulatae by preparative-HPLC and HSCCC.SEPARATION SCIENCE AND TECHNOLOGY,51(5),807-815.
MLA	Ye, Xiaolan,et al."Preparative separation of nine flavonoids from Pericarpium Citri Reticulatae by preparative-HPLC and HSCCC".SEPARATION SCIENCE AND TECHNOLOGY 51.5(2016):807-815.