Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging

	Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging
	Gao,Jing; Wang,Feng; Liu,Yanhou; Cai,Mingjun; Xu,Haijiao; Jiang,Junguang; Wang,Hongda
刊名	scientific reports
	2015
卷号	5 页码:9045
关键词	OPTICAL RECONSTRUCTION MICROSCOPY TYROSINE PHOSPHORYLATION TRANSCRIPTIONAL CONTROL FLUORESCENCE MICROSCOPY DIFFRACTION-LIMIT IFN-GAMMA PROTEIN ACTIVATION SIGNAL TRANSLOCATION
通讯作者	wang,hd
英文摘要	signal transducers and activators of transcription (stats) can transduce cytokine signals and regulate gene expression. the cellular localization and nuclear trafficking of stat1, a representative of the stat family with multiple transcriptional functions, is tightly related with transcription process, which usually happens in the interphase of the cell cycle. however, these priority questions regarding stat1 distribution and localization at the different cell-cycle stages remain unclear. by using direct stochastic optical reconstruction microscopy (dstorm), we found that the nuclear expression level of stat1 increased gradually as the cell cycle carried out, especially after egf stimulation. furthermore, stat1 formed clusters in the whole cell during the cell cycle, with the size and the number of clusters also increasing significantly from g1 to g2 phase, suggesting that transcription and other cell-cycle related activities can promote stat1 to form more and larger clusters for fast response to signals. our work reveals that the cellular localization and clustering distribution of stat1 are associated with the cell cycle, and further provides an insight into the mechanism of cell-cycle regulated stat1 signal transduction.
收录类别	SCI
语种	英语
WOS记录号	WOS:000351144900022
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64847]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Gao,Jing,Wang,Feng,Liu,Yanhou,et al. Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging[J]. scientific reports,2015,5:9045.
APA	Gao,Jing.,Wang,Feng.,Liu,Yanhou.,Cai,Mingjun.,Xu,Haijiao.,...&Wang,Hongda.(2015).Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging.scientific reports,5,9045.
MLA	Gao,Jing,et al."Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging".scientific reports 5(2015):9045.