Guanidinated Thiourea-Decorated Polyethylenimines for Enhanced Membrane Penetration and Efficient siRNA Delivery

	Guanidinated Thiourea-Decorated Polyethylenimines for Enhanced Membrane Penetration and Efficient siRNA Delivery
	Li,Yuce; Tian,Huayu; Ding,Jianxun; Lin,Lin; Chen,Jie; Gao,Shiqian; Chen,Xuesi
刊名	advanced healthcare materials
	2015
卷号	4 期号:9 页码:1369-1675
关键词	NONVIRAL GENE DELIVERY TAT-FUSION PROTEINS POLY(ASPARTATE-G-OEI) COPOLYMERS DRUG-DELIVERY IN-VITRO NANOPARTICLES TRANSFECTION PEPTIDES POLYMERS VECTORS
通讯作者	tian,hy
英文摘要	rna interference (rnai) provides the promising treatments of gene-related diseases while hindered by the lack of highly efficient delivery platform with low cytotoxicity. moreover, the intracellular fates of nonviral gene carriers are closely related to their internalization pathway, and eventually influence their rnai efficiency. herein, a series of guanidinated thiourea-modified polyethylenimines (pei-mtu-gs) are synthesized and utilized as the efficient carriers of small interfering rna (sirna) with up to 71.6% inhibition of luciferase activity in the luciferase-expressing cell lines (i.e., hela/luc cells). the introduction of noncationic hydrogen bond donors, that is, thiourea groups, provides the carriers with much lower cytotoxicities and relatively looser complex structures that facilitate the intracellular release of sirnas. furthermore, the multiguanidino structures endow the pei-mtu-g/sirna complexes with the ability to directly penetrate cell membrane, which facilitates the cellular internalization while avoiding them suffering from the rigorous lysosomes. the results demonstrate pei-mtu35-gs as promising sirna carriers for further gene therapy.
收录类别	SCI
语种	英语
WOS记录号	WOS:000356834000012
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64819]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li,Yuce,Tian,Huayu,Ding,Jianxun,et al. Guanidinated Thiourea-Decorated Polyethylenimines for Enhanced Membrane Penetration and Efficient siRNA Delivery[J]. advanced healthcare materials,2015,4(9):1369-1675.
APA	Li,Yuce.,Tian,Huayu.,Ding,Jianxun.,Lin,Lin.,Chen,Jie.,...&Chen,Xuesi.(2015).Guanidinated Thiourea-Decorated Polyethylenimines for Enhanced Membrane Penetration and Efficient siRNA Delivery.advanced healthcare materials,4(9),1369-1675.
MLA	Li,Yuce,et al."Guanidinated Thiourea-Decorated Polyethylenimines for Enhanced Membrane Penetration and Efficient siRNA Delivery".advanced healthcare materials 4.9(2015):1369-1675.