Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy

	Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy
	Yuan, Hongbo 1,2; Qi, Junjie 1; Xing, Chengfen 1; An, Hailong 1; Niu, Ruimin 1; Zhan, Yong 1; Fan, Yibing 1; Yan, Wenmin 1; Li, Ruihua 1; Wang, Bing 3
刊名	ADVANCED FUNCTIONAL MATERIALS
	2015-07-22
卷号	25 期号:28 页码:4412-4418
关键词	calmodulin conformation changes cationic-conjugated polymers graphene oxide
英文摘要	The conformation of calmodulin (CaM) changes from closed configuration to open one, converting to a claviform dumbbell-shaped biomolecule upon Ca2+-binding. A hybrid probe of graphene oxide (GO) cationic conjugated polymer for detection of the conformation transition of CaM by using FRET technique is demonstrated. The stronger hydrophobic interaction and weaker electrostatic repulsion leads to more CaM adsorption to the surface of GO upon binding with Ca2+ than that of CaM in the absence of Ca2+ (apoCaM), resulting in much farther proximity between poly[(9,9-bis(6-N,N,N-trimethylammonium)hexyl)-fluorenylene phenylene dibromide] (PFP) and green fluorescent protein labeled at the N-terminus of CaM and therefore much weaker FRET efficiency for PFP/Ca2+/CaM in comparison with that of PFP/apoCaM in the presence of GO. Notably, the assembly of CaM with GO is quantitatively and reversibly controlled by Ca2+ ions.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/27772]
专题	化学研究所_有机固体实验室
作者单位	1.Hebei Univ Technol, Inst Biophys, Key Lab Hebei Prov Mol Biophys, Tianjin 300401, Peoples R China 2.Hebei Univ Technol, Sch Mat Sci & Engn, Tianjin 300130, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Hongbo,Qi, Junjie,Xing, Chengfen,et al. Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy[J]. ADVANCED FUNCTIONAL MATERIALS,2015,25(28):4412-4418.
APA	Yuan, Hongbo.,Qi, Junjie.,Xing, Chengfen.,An, Hailong.,Niu, Ruimin.,...&Wang, Shu.(2015).Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy.ADVANCED FUNCTIONAL MATERIALS,25(28),4412-4418.
MLA	Yuan, Hongbo,et al."Graphene-Oxide-Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy".ADVANCED FUNCTIONAL MATERIALS 25.28(2015):4412-4418.