Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells

	Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells
	Zhu, Xiuzhen 1; Chen, Yi 1,2
刊名	AMINO ACIDS
	2015-08-01
卷号	47 期号:8 页码:1673-1674
关键词	Dielectrophoretic and electrophoretic mobilities Human red blood cells Simultaneous determination
英文摘要	Electrophoresis and dielectrophoresis of cells can reveal many distinct cellular properties but are often conducted separately. Herein a simultaneous strategy was proposed, and a simple method was established by making cells migrate through a cross channel under a micro video for real-time observation. The experiment can be performed within 0.044-1 s. In combination with digital calculation based on electromagnetic theory, the method was validated to be applicable to the determination of electrophoretic and dielectrophoretic mobilities, mu(EP) and mu(DEP), of human blood erythrocytes, giving mu(EP) = -(0.87 +/- 0.16) x10(-4) cm(2).V-1.s(-1) and mu(DEP)=-(4.5 +/- 1.3) x10(-8) cm(4).V-2.s(-1) by vector decomposition, or mu(EP)=-(0.89 +/- 0.14) x10(-4) cm(2).V-1.s(-1) and mu(DEP) =-(4.6 +/- 1.2) x10(-8) cm(4).V-2.s(-1) by least squares fitting, all agreeing with published data. Hydrodynamic and EOFs were eliminated for better measurement. It was found that the location of cells had a serious impact on the measurement precision, and the upstream of the cross channel along the electric field was chosen for precise measurement. The method is also extendable to the study of other cells and particles.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/27844]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Xiuzhen,Chen, Yi. Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells[J]. AMINO ACIDS,2015,47(8):1673-1674.
APA	Zhu, Xiuzhen,&Chen, Yi.(2015).Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells.AMINO ACIDS,47(8),1673-1674.
MLA	Zhu, Xiuzhen,et al."Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells".AMINO ACIDS 47.8(2015):1673-1674.