(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 研究所（批量导入）

	(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study
	Fu, Yunbin 1,2; Wang, Mengyu 1; Wang, Wenxia 1; Tuo, Yaqin 3; Guo, Zhimou 4; Du, Yuguang 5; Yin, Heng 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2015-11-01
卷号	81 期号:NOV 页码:1069-1073
关键词	(1 -> 3)-beta-D-Glucan oligosaccharides Monomer Degree of polymerization Hydrogen peroxide Tobacco
ISSN号	0141-8130
英文摘要	In order to produce highly purified (1 -> 3)-beta-D-glucan oligosaccharides ((1 -> 3)-beta-D-GOS) monomers, a hydrophilic interaction liquid chromatography (HILIC) system with X-Amide stationary phase was performed. Nine (1 -> 3)-beta-D-GOS monomers with degree of polymerization (DP) from 2 to 10 were successfully separated. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) demonstrated that these monomers were with high purity. Furthermore, a hydrogen peroxide (H2O2) online detection method was established to monitor H2O2 releases in tobacco cells. This is the first report on nine consecutive (1 -> 3)-beta-D-GOS monomers purification and its effect upon H2O2-releasing in plants. Itwas found that (1 -> 3)-beta-D-GOS monomers with higher DP induced stronger defense responses in plants, which will pave the way for elucidating the relationship between (1 -> 3)-beta-D-GOS and biological activities. (C) 2015 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
关键词[WOS]	TOBACCO SUSPENSION CELLS ; BETA-1,3 GLUCAN SULFATE ; DEFENSE RESPONSES ; PLASMOPARA-VITICOLA ; RESISTANCE ; GRAPEVINE ; DEATH ; OLIGOGALACTURONIDES ; CHROMATOGRAPHY ; SEPARATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000367408300133
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/20251]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Grp, Dalian 116023, Liaoning, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Dalian Glycobio Co Ltd, Dalian 116023, Liaoning, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Separat Mat & Separat Method Grp, Dalian 116023, Liaoning, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Fu, Yunbin,Wang, Mengyu,Wang, Wenxia,et al. (1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,81(NOV):1069-1073.
APA	Fu, Yunbin.,Wang, Mengyu.,Wang, Wenxia.,Tuo, Yaqin.,Guo, Zhimou.,...&Yin, Heng.(2015).(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,81(NOV),1069-1073.
MLA	Fu, Yunbin,et al."(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 81.NOV(2015):1069-1073.